2016年8月15日
Comparative Evaluation of Real-Time PCR Methods for Human Noroviruses in Wastewater and Human Stool
PLOS ONE
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- 巻
- 11
- 号
- 8
- 開始ページ
- e0160825
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1371/journal.pone.0160825
- 出版者・発行元
- PUBLIC LIBRARY SCIENCE
Selecting the best quantitative PCR assay is essential to detect human norovirus genome effectively from clinical and environmental samples because no cell lines have been developed to propagate this virus. The real-time PCR methods for noroviruses GI (4 assays) and GII (3 assays) were evaluated using wastewater (n = 70) and norovirus-positive stool (n = 77) samples collected in Japan between 2012 and 2013. Standard quantitative PCR assays recommended by the U.S. Environmental Protection Agency, International Organization for Standardization, and Ministry of Health, Labour and Welfare, Japan, together with recently reported assays were included. Significant differences in positive rates and quantification cycles were observed by non-parametric analysis. The present study identifies the best assay for norovirus GI and GII to amplify norovirus genomes efficiently.
- リンク情報
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- DOI
- https://doi.org/10.1371/journal.pone.0160825
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/27525654
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000381471100022&DestApp=WOS_CPL
- URL
- http://orcid.org/0000-0001-7418-869X
- ID情報
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- DOI : 10.1371/journal.pone.0160825
- ISSN : 1932-6203
- ORCIDのPut Code : 26062881
- PubMed ID : 27525654
- Web of Science ID : WOS:000381471100022