論文

査読有り
2016年1月

Changes in ovarian gene expression profiles and plasma hormone levels in maturing European eel (Anguilla anguilla); Biomarkers for broodstock selection

GENERAL AND COMPARATIVE ENDOCRINOLOGY
  • Erik Burgerhout
  • ,
  • Yuki Minegishi
  • ,
  • Sebastiaan A. Brittijn
  • ,
  • Danielle L. de Wijze
  • ,
  • Christiaan V. Henkel
  • ,
  • Hans J. Jansen
  • ,
  • Herman P. Spaink
  • ,
  • Ron P. Dirks
  • ,
  • Guido E. E. J. M. van den Thillart

225
開始ページ
185
終了ページ
196
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.ygcen.2015.08.006
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Complete sexual maturation of European eels (Anguilla anguilla) in captivity can only be achieved via injections with gonadotropins. For female eels this procedure takes 4-6 months and the response ranges from "unresponsive" to final maturation and ovulation. Reproductive success could be significantly increased via early selection of responders based on predictive markers and minimally invasive sampling methods. To get a better understanding of the genetic background of ovarian maturation of the European eel we performed a pilot deep-sequencing transcriptome analysis of ovarian tissue derived from a yellow eel, a prepubertal silver eel and a post-spawning matured eel. Two key players in steroidogenesis were strongly correlated with advanced sexual maturation, namely P450c17 and liver receptor homolog-1, suggesting that blood plasma steroids might qualify as minimally invasive markers for early detection of responders. Since the predictive value of plasma sex steroid levels for final maturation of the European eel had not yet been carefully examined, we performed an extensive artificial maturation trial. Farmed silver eels were treated with pituitary extracts and sampled at multiple time intervals. Expression of steroidogenesis-related genes in ovarian tissue of responding and non-responding eels after four weekly injections with pituitary extract was compared using a custom-built microarray and RNAseq. Increased expression of 17 beta-hsd1 was strongly linked to sexual maturation. Blood plasma levels of sex steroids were measured using ELISAs. We show that a 2.5-fold increase in blood-plasma estradiol level after 4 weekly pituitary extract injections is a strong predictor of final sexual maturation of female European eel. (C) 2015 Elsevier Inc. All rights reserved.


リンク情報
DOI
https://doi.org/10.1016/j.ygcen.2015.08.006
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000366953000020&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.ygcen.2015.08.006
  • ISSN : 0016-6480
  • eISSN : 1095-6840
  • Web of Science ID : WOS:000366953000020

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