2006年9月
Arachidonic acid can function as a signaling modulator by activating the TRPM5 cation channel in taste receptor cells
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
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- 巻
- 1761
- 号
- 9
- 開始ページ
- 1078
- 終了ページ
- 1084
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bbalip.2006.07.005
- 出版者・発行元
- ELSEVIER SCIENCE BV
Vertebrate sensory cells such as vomeronasal neurons and Drosophila photoreceptor cells use TRP channels to respond to exogenous stimuli. In mammalian taste cells, bitter and sweet substances as well as some amino acids are received by G protein-coupled receptors (T2Rs or T1Rs). As a result of activation of G protein and phospholipase C beta 2, the TPPM5 channel is activated. Intracellular Ca2+ is known to be a TRPM5 activator, but the participation of lipid activators remains unreported. To clarify the effect of arachidonic acid on TRPM5 in taste cells, we investigated the expression profile of a series of enzymes involved in controlling the intracellular free arachidonic acid level, with the result that in a subset of taste bud cells, monoglyceride lipase (MGL) and cyclooxygenase-2 (COX-2) are expressed as well as the previously reported group IIA phospholipase A(2) (PLA(2)-IIA). Double-labeling analysis revealed that MGL, COX-2 and PLA(2)-IIA are co-expressed in some cells that express TRPM5. We then investigated whether arachidonic acid activates TRPM5 via a heterologous expression system in HEK293 cells, and found that its activation occurred at 10 mu M arachidonic acid. These results strongly suggest the possibility that arachidonic acid acts as a modulator of TRPN15 in taste signaling pathways. (c) 2006 Elsevier B.V. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.bbalip.2006.07.005
- ISSN : 1388-1981
- eISSN : 1879-2618
- Web of Science ID : WOS:000241273700011