論文

査読有り 国際誌
2019年4月7日

Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine.

Cancers
  • Tomohiro Osaki
  • ,
  • Inoru Yokoe
  • ,
  • Yuji Sunden
  • ,
  • Urara Ota
  • ,
  • Tomoki Ichikawa
  • ,
  • Hideo Imazato
  • ,
  • Takuya Ishii
  • ,
  • Kiwamu Takahashi
  • ,
  • Masahiro Ishizuka
  • ,
  • Tohru Tanaka
  • ,
  • Liming Li
  • ,
  • Masamichi Yamashita
  • ,
  • Yusuke Murahata
  • ,
  • Takeshi Tsuka
  • ,
  • Kazuo Azuma
  • ,
  • Norihiko Ito
  • ,
  • Tomohiro Imagawa
  • ,
  • Yoshiharu Okamoto

11
4
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/cancers11040495

5-Aminolevulinic acid (5-ALA), a commonly used photosensitizer in photodynamic detection (PDD) and therapy (PDT), is converted in situ to the established photosensitizer protoporphyrin IX (PpIX) via the heme biosynthetic pathway. To extend 5-ALA-PDT application, we evaluated the PpIX fluorescence induced by exogenous 5-ALA in various veterinary tumors and treated canine and feline tumors. 5-ALA-PDD sensitivity and specificity in the whole sample group for dogs and cats combined were 89.5 and 50%, respectively. Notably, some small tumors disappeared upon 5-ALA-PDT. Although single PDT application was not curative, repeated PDT+/-chemotherapy achieved long-term tumor control. We analyzed the relationship between intracellular PpIX concentration and 5-ALA-PDT in vitro cytotoxicity using various primary tumor cells and determined the correlation between intracellular PpIX concentration and 5-ALA transporter and metabolic enzyme mRNA expression levels. 5-ALA-PDT cytotoxicity in vitro correlated with intracellular PpIX concentration in carcinomas. Ferrochelatase mRNA expression levels strongly negatively correlated with PpIX accumulation, representing the first report of a correlation between mRNA expression related to PpIX accumulation and PpIX concentration in canine tumor cells. Our findings suggested that the results of 5-ALA-PDD might be predictive for 5-ALA-PDT therapeutic effects for carcinomas, with 5-ALA-PDT plus chemotherapy potentially increasing the probability of tumor control in veterinary medicine.

リンク情報
DOI
https://doi.org/10.3390/cancers11040495
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30959982
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520946
ID情報
  • DOI : 10.3390/cancers11040495
  • PubMed ID : 30959982
  • PubMed Central 記事ID : PMC6520946

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