論文

査読有り 国際誌
2018年8月

Effects of TONS504-photodynamic therapy on mouse mammary tumor cells.

Oncology letters
  • Tomohiro Osaki
  • ,
  • Isao Sakata
  • ,
  • Yoshihiro Uto
  • ,
  • Masamichi Yamashita
  • ,
  • Yusuke Murahata
  • ,
  • Kazuo Azuma
  • ,
  • Takeshi Tsuka
  • ,
  • Norihiko Ito
  • ,
  • Tomohiro Imagawa
  • ,
  • Yoshiharu Okamoto

16
2
開始ページ
2078
終了ページ
2084
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3892/ol.2018.8887

In the present study, TONS504 (C51H58N8O5I2; molecular weight, 1,116.9), a novel cationic hydrophilic photosensitizer, was synthesized from protoporphyrin IX dimethyl ester through a five-step process according to a patented method for use in photodynamic therapy (PDT). The subcellular localization of TONS504 and the cytotoxic effects of TONS504-mediated PDT in the mouse mammary tumor EMT6 cell line were investigated. TONS504 was localized primarily in the lysosomes and partially in the mitochondria. The cytotoxic effects of TONS504-mediated PDT in the mouse mammary tumor EMT6 cell line were investigated using a WST8 assay and an Oxidative Stress kit. The cell viability values following treatment with 10 µg/ml TONS504 at light energies of 0, 1, 5 and 10 J/cm2 were 92.5, 101.8, 27.7 and 1.8%, respectively. The percentages of reactive oxygen species (ROS)(+) cells following the same treatment were 8.6, 8.5, 29.2 and 70.1%, respectively, whereas the percentages of apoptotic cells were 7.1, 5.6, 24.8 and 48.7%, respectively. The percentages of ROS(+) and apoptotic cells in the group subjected to TONS504-mediated PDT increased in a manner dependent on the TONS504 concentration and light energy. Further studies are required to evaluate the in vivo pharmacokinetics, tissue distribution and photodynamic effects of TONS504.

リンク情報
DOI
https://doi.org/10.3892/ol.2018.8887
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30008903
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036337
ID情報
  • DOI : 10.3892/ol.2018.8887
  • PubMed ID : 30008903
  • PubMed Central 記事ID : PMC6036337

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