論文

査読有り 国際誌
2017年7月

Metformin enhances the cytotoxicity of 5-aminolevulinic acid-mediated photodynamic therapy in vitro.

Oncology letters
  • Tomohiro Osaki
  • ,
  • Inoru Yokoe
  • ,
  • Kiwamu Takahashi
  • ,
  • Katsushi Inoue
  • ,
  • Masahiro Ishizuka
  • ,
  • Tohru Tanaka
  • ,
  • Kazuo Azuma
  • ,
  • Yusuke Murahata
  • ,
  • Takeshi Tsuka
  • ,
  • Norihiko Itoh
  • ,
  • Tomohiro Imagawa
  • ,
  • Yoshiharu Okamoto

14
1
開始ページ
1049
終了ページ
1053
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3892/ol.2017.6237

The biguanide metformin is a drug widely used for the treatment of type 2 diabetes. Metformin enhances the cytotoxicity of chemotherapy by promoting the adenosine monophosphate-activated protein kinase (AMPK) autophagy signaling pathway. Photodynamic therapy (PDT) with 5-aminolevulinic acid (5-ALA), a precursor of protoporphyrin IX (PpIX), leads to apoptosis when PpIX accumulates in the mitochondria, and also leads to autophagy through activation of AMPK. In the present study, the effect of metformin in combination with 5-ALA-PDT was evaluated in vitro in KLN205 lung cancer cells. At a fluence of 5 J/cm2, 5-ALA-PDT in combination with 5 mM metformin exhibited significantly increased cytotoxicity compared with that observed with 0 and 0.1 mM metformin (P=0.0197 and P=0.0423, respectively). The cells treated with 5-ALA-PDT and metformin exhibited condensation of nuclear chromatin and the presence of autophagosomes. These results indicate that apoptosis and autophagy occur in KLN205 cells following combined treatment with 5-ALA-PDT and metformin. The results from the present study are the first to indicate, to the best of our knowledge, that metformin potentiates the efficacy of 5-ALA-PDT.

リンク情報
DOI
https://doi.org/10.3892/ol.2017.6237
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28693272
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494612
ID情報
  • DOI : 10.3892/ol.2017.6237
  • PubMed ID : 28693272
  • PubMed Central 記事ID : PMC5494612

エクスポート
BibTeX RIS