論文

査読有り 国際誌
2017年3月27日

Artesunate Enhances the Cytotoxicity of 5-Aminolevulinic Acid-Based Sonodynamic Therapy against Mouse Mammary Tumor Cells In Vitro.

Molecules (Basel, Switzerland)
  • Tomohiro Osaki
  • ,
  • Yoshihiro Uto
  • ,
  • Masahiro Ishizuka
  • ,
  • Tohru Tanaka
  • ,
  • Nobuyasu Yamanaka
  • ,
  • Tsukasa Kurahashi
  • ,
  • Kazuo Azuma
  • ,
  • Yusuke Murahata
  • ,
  • Takeshi Tsuka
  • ,
  • Norihiko Itoh
  • ,
  • Tomohiro Imagawa
  • ,
  • Yoshiharu Okamoto

22
4
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/molecules22040533

Sonodynamic therapy (SDT) kills tumor cells through the synergistic effects of ultrasound (US) and a sonosensitizer agent. 5-Aminolevulinic acid (5-ALA) has been used as a sonodynamic sensitizer for cancer treatment. However, studies have shown that 5-ALA-based SDT has limited efficacy against malignant tumors. In this study, we examined whether artesunate (ART) could enhance the cytotoxicity of 5-ALA-based SDT against mouse mammary tumor (EMT-6) cells in vitro. In the ART, ART + US, ART + 5-ALA, and ART + 5-ALA + US groups, the cell survival rate correlated with ART concentration, and decreased with increasing concentrations of ART. Morphologically, many apoptotic and necrotic cells were observed in the ART + 5-ALA + US group. The percentage of reactive oxygen species-positive cells in the ART + 5-ALA + US group was also significantly higher than that in the 5-ALA group (p = 0.0228), and the cell death induced by ART + 5-ALA + US could be inhibited by the antioxidant N-acetylcysteine. These results show that ART offers great potential in enhancing the efficacy of 5-ALA-based SDT for the treatment of cancer. However, these results are only based on in vitro studies, and further in vivo studies are required.

リンク情報
DOI
https://doi.org/10.3390/molecules22040533
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28346389
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154000
ID情報
  • DOI : 10.3390/molecules22040533
  • PubMed ID : 28346389
  • PubMed Central 記事ID : PMC6154000

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