論文

査読有り 国際誌
2017年6月

Effects of photodynamic therapy with talaporfin sodium on squamous cell carcinoma and sarcoma cells.

Photodiagnosis and photodynamic therapy
  • Tomohiro Osaki
  • ,
  • Yuki Kawase
  • ,
  • Hiroshi Iseki
  • ,
  • Shinji Kishimoto
  • ,
  • Soko Ikuta
  • ,
  • Yoshihiro Muragaki
  • ,
  • Masamichi Yamashita
  • ,
  • Kazuo Azuma
  • ,
  • Yusuke Murahata
  • ,
  • Takeshi Tsuka
  • ,
  • Norihiko Itoh
  • ,
  • Tomohiro Imagawa
  • ,
  • Yoshiharu Okamoto

18
開始ページ
213
終了ページ
220
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.pdpdt.2017.02.007

BACKGROUND: Photodynamic therapy (PDT) uses a photosensitizer and light to destroy abnormal cells. Talaporfin sodium (NPe6) is a second-generation photosensitizer. METHODS: We evaluated the toxic effects of different combinations of laser and NPe6 doses on squamous cell carcinoma (KLN205) and sarcoma (Meth A) cell lines. The cells were incubated with 0, 5, 10, or 30μg/mL NPe6 for 24h. The cells were then irradiated with 0, 5, 15, or 30mW/cm2 of laser power, and 0, 1, 5, 10, or 20J/cm2 of laser energy. Cell viability was evaluated after 24h. We also evaluated the cytotoxic effects of continuous wave or square-wave modulated laser irradiations (2, 5, or 10Hz, 50% duty) on Meth A cells. RESULTS: The median lethal doses of NPe6 against the KLN205 and Meth A cells after treatment at a fluence rate of 15mW/cm2 and a light dose of 20J/cm2 were 18.6 and 5.0μg/mL, respectively. Meth A cells were more sensitive to PDT than KLN205 cells. There was no significant difference between the effects of continuous wave and square-wave modulated lasers on Meth A cell viability. CONCLUSIONS: NPe6 PDT induced cell death in a dose-dependent manner in KLN205 and Meth A cells. More work is required to evaluate the cytotoxic effects of square-wave modulated laser therapy at low light doses.

リンク情報
DOI
https://doi.org/10.1016/j.pdpdt.2017.02.007
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28232036
ID情報
  • DOI : 10.1016/j.pdpdt.2017.02.007
  • PubMed ID : 28232036

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