論文

査読有り 国際誌
2016年9月30日

Establishment of a canine mammary gland tumor cell line and characterization of its miRNA expression.

Journal of veterinary science
  • Tomohiro Osaki
  • ,
  • Yuji Sunden
  • ,
  • Akihiko Sugiyama
  • ,
  • Kazuo Azuma
  • ,
  • Yusuke Murahata
  • ,
  • Takeshi Tsuka
  • ,
  • Norihiko Ito
  • ,
  • Tomohiro Imagawa
  • ,
  • Yoshiharu Okamoto

17
3
開始ページ
385
終了ページ
90
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.4142/jvs.2016.17.3.385

Canine mammary gland tumors (CMGTs), which are the most common neoplasms in sexually intact female dogs, have been suggested as a model for studying human breast cancer because of several similarities, including relative age of onset, risk factors, incidence, histological and molecular features, biological behavior, metastatic pattern, and responses to therapy. In the present study, we established a new cell line, the SNP cell line, from a CMGT. A tumor formed in each NOD.CB17-Prkdc(scid)/J mouse at the site of subcutaneous SNP cell injection. SNP cells are characterized by proliferation in a tubulopapillary pattern and are vimentin positive. Moreover, we examined miRNA expression in the cultured cells and found that the expression values of miRNA-143 and miRNA-138a showed the greatest increase and decrease, respectively, of all miRNAs observed, indicating that these miRNAs might play a significant role in the malignancy of SNP cells. Overall, the results of this study indicate that SNP cells might serve as a model for future genetic analysis and clinical treatments of human breast tumors.

リンク情報
DOI
https://doi.org/10.4142/jvs.2016.17.3.385
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26726024
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037307
ID情報
  • DOI : 10.4142/jvs.2016.17.3.385
  • PubMed ID : 26726024
  • PubMed Central 記事ID : PMC5037307

エクスポート
BibTeX RIS