論文

査読有り 国際誌
2020年3月20日

Off-Loading Mechanism of Products in Polyunsaturated Fatty Acid Synthases.

ACS chemical biology
  • Shohei Hayashi
  • ,
  • Yasushi Ogasawara
  • ,
  • Yasuharu Satoh
  • ,
  • Chitose Maruyama
  • ,
  • Yoshimitsu Hamano
  • ,
  • Tohru Dairi

15
3
開始ページ
651
終了ページ
656
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/acschembio.0c00075
出版者・発行元
American Chemical Society ({ACS})

Marine microorganisms de novo biosynthesize polyunsaturated fatty acids such as docosahexaenoic acid and eicosapentaenoic acid by polyunsaturated fatty acid (PUFA) synthases composed of three or four polypeptides in a manner similar to fatty acid synthases (FASs). FASs usually possess thioesterase (TE) domains to release free fatty acids from acyl carrier protein (ACP)-tethered intermediates. Here, we investigated the off-loading mechanism with microalgal and bacterial PUFA synthases through in vivo and in vitro experiments. The in vitro experiments with acyltransferase (AT)-like domains and acyl-ACP substrates clearly demonstrated that the AT-like domains catalyzed the hydrolysis of acyl-ACPs to yield free fatty acids.

リンク情報
DOI
https://doi.org/10.1021/acschembio.0c00075
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32105442

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