2020年3月20日
Off-Loading Mechanism of Products in Polyunsaturated Fatty Acid Synthases.
ACS chemical biology
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- 巻
- 15
- 号
- 3
- 開始ページ
- 651
- 終了ページ
- 656
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/acschembio.0c00075
- 出版者・発行元
- American Chemical Society ({ACS})
Marine microorganisms de novo biosynthesize polyunsaturated fatty acids such as docosahexaenoic acid and eicosapentaenoic acid by polyunsaturated fatty acid (PUFA) synthases composed of three or four polypeptides in a manner similar to fatty acid synthases (FASs). FASs usually possess thioesterase (TE) domains to release free fatty acids from acyl carrier protein (ACP)-tethered intermediates. Here, we investigated the off-loading mechanism with microalgal and bacterial PUFA synthases through in vivo and in vitro experiments. The in vitro experiments with acyltransferase (AT)-like domains and acyl-ACP substrates clearly demonstrated that the AT-like domains catalyzed the hydrolysis of acyl-ACPs to yield free fatty acids.
- リンク情報
- ID情報
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- DOI : 10.1021/acschembio.0c00075
- ORCIDのPut Code : 69903023
- PubMed ID : 32105442