論文

査読有り 国際誌
2020年1月24日

Lipopolysaccharide (LPS) inhibits ectopic bone formation induced by bone morphogenetic protein-2 and TGF-β1 through IL-1β production.

Journal of Oral Biosciences
  • Akifumi Matsumoto
  • ,
  • Masamichi Takami
  • ,
  • Eri Urano
  • ,
  • Tomoya Nakamachi
  • ,
  • Kentaro Yoshimura
  • ,
  • Atsushi Yamada
  • ,
  • Tetsuo Suzawa
  • ,
  • Yoichi Miyamoto
  • ,
  • Kazuyoshi Baba
  • ,
  • Ryutaro Kamijo

記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.job.2020.01.004

OBJECTIVES: In order to gain new insight into bacterial infection during bone-regenerative treatment using bone morphogenetic proteins (BMPs), we examined the effects of lipopolysaccharide (LPS) on ectopic bone formation induced by BMP-2 and transforming growth factor (TGF)-β1 in mice. METHODS: We implanted collagen sponges containing BMP-2, TGF-β1, and various amounts of LPS into mouse muscle tissues. Lump-like masses in which ectopic bones developed in mice were processed for microcomputed tomography, DNA microarray, reverse-transcription PCR, and histological analyses. RESULTS: LPS treatment caused a dose-dependent reduction in the volume of ectopic bone. The total volume of ectopic bone induced by BMP-2 + TGF-β1 treatment was reduced by more than 75% in the presence of LPS. Histological analysis of the ectopic bone tissues revealed a significant reduction in total bone volume and bone volume/total volume in response to LPS. LPS treatment significantly increased the osteoblast number and osteoid volume, while the osteoclast number did not change. Since LPS induced production of TNF-α and IL-1β in lump-like masses, we implanted collagen sponges containing BMP-2 and TGF-β1 with or without LPS into TNF-α- or IL-1α/β-deficient mice. LPS treatment reduced the volume of ectopic bones in TNF-α-deficient mice but not in IL-1α/β-deficient mice. Furthermore, collagen sponges containing IL-1β reduced ectopic bone formation by BMP-2 and TGF-β1 in wild-type mice to the same extent as LPS treatment did. CONCLUSIONS: LPS suppresses the ectopic bone formation induced by BMP-2 and TGF-β1 through IL-1β production.

リンク情報
DOI
https://doi.org/10.1016/j.job.2020.01.004
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31987892

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