2019年11月1日
Synthesis and evaluation of a radioiodinated BODIPY derivative as a thiol-labeling agent
Journal of Labelled Compounds and Radiopharmaceuticals
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- 巻
- 62
- 号
- 13
- 開始ページ
- 885
- 終了ページ
- 891
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/jlcr.3809
© 2019 John Wiley & Sons, Ltd. Labeling agents with radioisotopes or fluorescent dyes are useful for investigating the biodistributions of biologically active proteins and peptides. Compared with molecular imaging with a single modality, dual imaging probes provide complementary information for each modality. The development of a dual radioisotope/fluorescence agent for protein labeling would thus be valuable for both preclinical and clinical applications. In this study, we designed and synthesized a radioiodinated BODIPY derivative (BODIPY-ML) with a maleimide group as a thiol-labeling agent. In the presence of N-chlorosuccinimide and 1% acetic acid, [125I]BODIPY-ML was successfully obtained at a radiochemical yield of 42%. In conjugation studies, model proteins including RGD peptides and anti-HER2 VHH were successfully labeled with BODIPY-ML via covalent bonds. The results demonstrated the feasibility of the radioiodinated BODIPY as a dual-labeling agent via thiol groups.
- リンク情報
- ID情報
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- DOI : 10.1002/jlcr.3809
- ISSN : 0362-4803
- eISSN : 1099-1344
- PubMed ID : 31677180
- SCOPUS ID : 85074827449