1995年8月
THE INTERACTION OF REPC INITIATOR WITH ITERONS IN THE REPLICATION OF THE BROAD-HOST-RANGE PLASMID RSF1010
NUCLEIC ACIDS RESEARCH
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- 巻
- 23
- 号
- 16
- 開始ページ
- 3295
- 終了ページ
- 3300
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/nar/23.16.3295
- 出版者・発行元
- OXFORD UNIV PRESS UNITED KINGDOM
The replication origin of the broad host-range plasmid RSF1010 contains 3.5 copies of a 20mer iteron sequence that bind specifically to the plasmid-encoded initiator, RepC. Here we demonstrated that even a single iteron was bent upon binding of RepC. Moreover, the bending angle seems to become larger along with the increment of the number of iterons. In a mutational analysis of the iteron sequence, we isolated seven kinds of base-substitution mutants of iterons, and estimated the replication activity of these mutants in vivo. We found that each of the subsections in the 20mer iteron sequence made a distinct contribution to the initiation of RSF1010 DNA replication. With the binding assay of RepC and mutated iterons in vitro, we found that the formation of a productive RepC-iteron complex was required for the initiation of plasmid DNA replication.
- リンク情報
- ID情報
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- DOI : 10.1093/nar/23.16.3295
- ISSN : 0305-1048
- PubMed ID : 7667106
- Web of Science ID : WOS:A1995RU26900028