論文

査読有り 国際誌
2019年7月

Oxidative DNA Damage and Apoptosis Induced by Aclarubicin, an Anthracycline: Role of Hydrogen Peroxide and Copper.

Anticancer research
  • Hideki Mizutani
  • ,
  • Yuka Hayashi
  • ,
  • Miyabi Hashimoto
  • ,
  • Masanori Imai
  • ,
  • Yoshimi Ichimaru
  • ,
  • Yuki Kitamura
  • ,
  • Kenji Ikemura
  • ,
  • Daisuke Miyazawa
  • ,
  • Kinya Ohta
  • ,
  • Yoshiaki Ikeda
  • ,
  • Tohru Maeda
  • ,
  • Masae Yoshikawa
  • ,
  • Yusuke Hiraku
  • ,
  • Shosuke Kawanishi

39
7
開始ページ
3443
終了ページ
3451
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.21873/anticanres.13490

BACKGROUND/AIM: This study aimed to investigate aclarubicin (ACR)-induced oxidative DNA damage and apoptosis. MATERIALS AND METHODS: ACR-induced apoptosis was analyzed using HL-60 leukemia cells and HP100 cells, hydrogen peroxide (H2O2)-resistant cells derived from HL-60 cells. ACR-induced DNA damage was analyzed using plasmid DNA. RESULTS: HL-60 cells were more sensitive to ACR than HP100 cells. In HP100 cells, DNA ladder formation and caspase-3/7 activity induced by ACR were suppressed or delayed in comparison to those in HL-60 cells. ACR-induced DNA damage occurred in the presence of Cu(II), and scavenger experiments showed that the reactive species causing DNA damage appeared to be generated from H2O2 and Cu(I). Moreover, we detected intracellular Cu(I) induced by ACR in HL-60 cells, using CopperGREEN™, a fluorescent probe for detection of Cu(I) ion specifically. CONCLUSION: ACR-induced DNA damage and apoptosis can be accounted for by the involvement of H2O2 and Cu(I).

リンク情報
DOI
https://doi.org/10.21873/anticanres.13490
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31262868

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