MISC

2019年6月

Real-Time Compact Identification System of Amplified Target RNA Based on a Filter-Free Multiple Wavelengths Sensor

2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
  • Yong Joon Choi
  • ,
  • Kazuhiro Takahashi
  • ,
  • Toshihiko Noda
  • ,
  • Tatsuya Iwata
  • ,
  • Hiromu Ishii
  • ,
  • Kazuaki Sawada

開始ページ
2251
終了ページ
2254
DOI
10.1109/TRANSDUCERS.2019.8808758

© 2019 IEEE. We proposed an identification system of amplified target ribonucleic acids (RNAs) without optical filters using a filter-free multiple wavelengths sensor. To detect fluorescence from transcription reverse transcription concerted reaction (TRC) combined with intercalation activating fluorescence probe (INAF), a compact system fabricated with optimized physical parameters of light source and sensor. The excitation light and fluorescence from reagent were successfully separated using the proposed parameters based on controlled the intensity of the excitation light and the potential depth of the sensor. We succeeded in identifying each target RNAs in less than 10 minutes without using an optical filter. The Filter-free multiple wavelengths sensor can be applied in the application of biotechnology and help to realize miniaturized bio-devices.

リンク情報
DOI
https://doi.org/10.1109/TRANSDUCERS.2019.8808758
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85071920786&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85071920786&origin=inward

エクスポート
BibTeX RIS