2017年10月
Cholesterol is required for stability and infectivity of influenza A and respiratory syncytial viruses
VIROLOGY
- ,
- ,
- ,
- 巻
- 510
- 号
- 開始ページ
- 234
- 終了ページ
- 241
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.virol.2017.07.024
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Cholesterol-rich lipid raft microdomains in the plasma membrane are considered to play a major role in the enveloped virus lifecycle. However, the functional role of cholesterol in assembly, infectivity and stability of respiratory RNA viruses is not fully understood. We previously reported that depletion of cellular cholesterol by cholesterol-reducing agents decreased production of human parainfluenza virus type 1 (hPIV1) particles by inhibiting virus assembly. In this study, we analyzed the role of cholesterol on influenza A virus (IAV) and respiratory syncytial virus (RSV) production. Unlike hPIV1, treatment of human airway cells with the agents did not decrease virus particle production. However, the released virions were less homogeneous in density and unstable. Addition of exogenous cholesterol to the released virions restored virus stability and infectivity. Collectively, these data indicate a critical role of cholesterol in maintaining IAV and RSV membrane structure that is essential for sustaining viral stability and infectivity.
- リンク情報
- ID情報
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- DOI : 10.1016/j.virol.2017.07.024
- ISSN : 0042-6822
- PubMed ID : 28750327
- Web of Science ID : WOS:000408400100026