2013年8月
Summer heat stress affects prostaglandin synthesis in the bovine oviduct
Reproduction
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- 巻
- 146
- 号
- 2
- 開始ページ
- 103
- 終了ページ
- 110
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1530/REP-12-0479
- 出版者・発行元
- BIOSCIENTIFICA LTD
Summer heat stress (HS) negatively affects reproductive functions, including prostaglandin (PG) F-2 alpha secretion in the endometrium, and decreases fertility in cattle. In the present study, we examined the effects of elevated temperatures on PG synthesis in oviductal epithelial cells. The epithelial cells obtained from the ampulla and isthmus of the oviduct were incubated at various temperatures (38.5, 39.5, 40.0, and 40.5 degrees C) for 24 h. In the ampulla, PGE(2) concentration was higher at 40.5 degrees C than at 38.5 degrees C, while PGF(2 alpha) production was not affected by the temperatures in this range. The expressions of microsomal PGE synthase 1 (PTGES (mPGES1)), cytosolic PGES (PTGES3 (cPGES)), and heat shock protein 90 (HSP90AA1 (HSP90)) mRNAs and proteins were higher at 40.5 degrees C than at 38.5 degrees C in the ampullary epithelial cells. Seasonal changes in the expressions of PGES and HSP90AA1 mRNAs in oviductal tissues were also investigated. The expressions of PTGES3 and HSP90AA1 mRNAs were higher in the ampullary tissues in summer than in winter. In summary, elevated temperatures stimulated PGE(2) production in the ampullary oviduct by increasing the expressions of PGESs and HSP90AA1, which can activate cPGES. The overall results suggest that HS upsets PG secretions and reduces oviductal smooth muscle motility, which in turn could decrease gamete/embryo transport through the oviduct.
- リンク情報
- ID情報
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- DOI : 10.1530/REP-12-0479
- ISSN : 1470-1626
- PubMed ID : 23704311
- Web of Science ID : WOS:000322366900005