論文

査読有り 筆頭著者 国際誌
2021年8月17日

Cdc42 is required for male germline niche development in mice.

Cell reports
  • Yoshifumi Mori
  • ,
  • Seiji Takashima
  • ,
  • Mito Kanatsu-Shinohara
  • ,
  • Zheng Yi
  • ,
  • Takashi Shinohara

36
7
開始ページ
109550
終了ページ
109550
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.celrep.2021.109550

Spermatogonial stem cells (SSCs) are maintained in a special microenvironment called a niche. However, much is unknown about components that constitute the niche. Here, we report that Cdc42 is essential for germline niche development. Sertoli cell-specific Cdc42-deficient mice showed normal premeiotic spermatogenesis. However, germ cells gradually disappeared during haploid cell formation and few germ cells remained in the mature testes. Spermatogonial transplantation experiments revealed a significant loss of SSCs in Cdc42-deficient testes. Moreover, Cdc42 deficiency in Sertoli cells downregulated GDNF, a critical factor for SSC maintenance. Cdc42-deficient Sertoli cells also exhibited lower nuclear MAPK1/3 staining. Inhibition of MAP2K1 or depletion of Pea15a scaffold protein downregulated GDNF expression. A screen of transcription factors revealed that Cdc42-deficient Sertoli cells downregulate DMRT1 and SOX9, both of which are critical for Sertoli cell development. These results indicate that Cdc42 is essential for niche function via MAPK1/3-dependent GDNF secretion.

リンク情報
DOI
https://doi.org/10.1016/j.celrep.2021.109550
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/34407418
ID情報
  • DOI : 10.1016/j.celrep.2021.109550
  • PubMed ID : 34407418

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