論文

査読有り
2003年10月

Systematic screening for signaling molecules expressed during somitogenesis by the signal sequence trap method

DEVELOPMENTAL BIOLOGY
  • A Tonegawa
  • ,
  • T Kasai
  • ,
  • Y Takahashi

262
1
開始ページ
32
終了ページ
50
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/S0012-1606(03)00327-0
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

We describe a systematic screening to search for molecules that act as an extracellular signal during somitogenesis in vertebrates. Somitogenesis, which gives rise to segmented structures of axial bones and muscles, is a consequence of cooperative morphogenetic movements caused by precisely regulated cell and tissue interactions. We employed a strategy that combined subtractive hybridization to enrich paraxial mesoderm/somite-specific cDNAs and the signal sequence trap method, which selects signal sequence-containing molecules. Ninety-two independent cDNAs found to possess a putative signal sequence or a transmembrane domain are presented with a data base accession number for each. These clones include cDNAs which were previously identified with a function characterized, cDNAs previously identified with an undetermined function, and also cDNAs with no similarity to known sequences. Among them, 16 clones exhibited peculiar patterns of expression in the presomitic mesoderm/somites revealed by whole-mount and section in situ hybridization techniques, with some clones also being expressed in the forming neural tube. This is the first report in which an elaborate strategy combining three different screening steps was employed to identify signaling molecules relevant to a particular morphogenetic process. (C) 2003 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/S0012-1606(03)00327-0
CiNii Articles
http://ci.nii.ac.jp/naid/80016237286
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/14512016
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000185616400003&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/S0012-1606(03)00327-0
  • ISSN : 0012-1606
  • CiNii Articles ID : 80016237286
  • PubMed ID : 14512016
  • Web of Science ID : WOS:000185616400003

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