論文

査読有り
2010年2月10日

Newly mutated putative-aminotransferase in nonpungent pepper (Capsicum annuum) results in biosynthesis of capsinoids, capsaicinoid analogues

Journal of Agricultural and Food Chemistry
  • Yoshiyuki Tanaka
  • ,
  • Munetaka Hosokawa
  • ,
  • Tetsuya Miwa
  • ,
  • Tatsuo Watanabe
  • ,
  • Susumu Yazawa

58
3
開始ページ
1761
終了ページ
1767
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/jf903282r
出版者・発行元
AMER CHEMICAL SOC

Capsinoids make up a group of nonpungent capsaicinoid analogues produced in Capsicum fruits. They have bioactivities similar to those of capsaicinoids such as suppression of fat accumulation and antioxidant activity. Because of their low pungency, they are more palatable ingredients in dietary supplements than capsaicinoids. We recently reported that capsinoid biosynthesis is caused by nonsense mutation in a putative aminotransferase gene (p-AMT) in a nonpungent cultivar CH-19 Sweet. Here we report on the screening of nonpungent germplasm that revealed a nonpungent cultivar Himo, which contains high levels of capsinoids. We have shown that Himo has a recessive allele of p-amt, which contains a mutation different from that of CH-19 Sweet. Sequence analysis of p-amt in Himo revealed that a single-nucleotide substitution results in one amino acid substitution from cysteine to arginine in the pyridoxal 5-phosphate binding domain. Genetic analysis using a cleaved amplified polymorphic sequence marker confirmed that the p-AMT genotype was precisely cosegregated with capsinoid biosynthesis and nonpungency. Himo will provide a new natural source of capsinoids. © 2009 American Chemical Society.

リンク情報
DOI
https://doi.org/10.1021/jf903282r
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/20025278
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000274269000054&DestApp=WOS_CPL
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=76449114642&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=76449114642&origin=inward
ID情報
  • DOI : 10.1021/jf903282r
  • ISSN : 0021-8561
  • eISSN : 1520-5118
  • PubMed ID : 20025278
  • SCOPUS ID : 76449114642
  • Web of Science ID : WOS:000274269000054

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