Papers

Feb 28, 2013

Expression of Toll-Like Receptor 4 in Glomerular Endothelial Cells under Diabetic Conditions.

Acta histochemica et cytochemica
  • Shunsuke Takata
  • ,
  • Yoshihiko Sawa
  • ,
  • Takanobu Uchiyama
  • ,
  • Hiroyuki Ishikawa

Volume
46
Number
1
First page
35
Last page
42
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1267/ahc.13002

Diabetic conditions promote glomerulosclerosis by mesangial cells but the mechanisms are not fully elucidated. The present study evaluated the expression of toll-like receptor 4 in glomerular endothelial cells in the streptozotocin (STZ)-induced type 1 diabetic mouse (ICR-STZ) and the type 2 diabetic KK/TaJcl mouse which were fed a high fat diet feed (KK/Ta-HF). In the ICR-STZ and KK/Ta-HF almost glomeruli were immunostained with anti-TLR4 but there was no glomerulus immunostained by ani-TLR4 in the control ICR and KK/Ta. Laser-scanning confocal microscopy showed that the TLR4-positive region did not coincide with the podoplanin-positive region but coincide with the PECAM-1- and VE-cadherin-positive regions in the glomeruli of the ICR-STZ and KK/Ta-HF. The in situ hybridization showed that almost signals for TLR4 mRNA were present in the glomerulus of the ICR-STZ and KK/Ta-HF to a stronger extent than in the control ICR and KK/Ta. These suggest that glomerular endothelial cells usually express the TLR4 gene and hyperglycemia in the diabetic condition induces the TLR4 protein expression in the glomerular capillary endothelial cells. Cytokine productions through the TLR signaling pathway in glomerular endothelial cells may allow mesangial cells to produce extracellular matrix proteins in the diabetic milieu.

Link information
DOI
https://doi.org/10.1267/ahc.13002
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23554538
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596605
ID information
  • DOI : 10.1267/ahc.13002
  • Pubmed ID : 23554538
  • Pubmed Central ID : PMC3596605

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