2017年10月
Peptide aptamer-assisted immobilization of green fluorescent protein for creating biomolecule-complexed carbon nanotube device
JAPANESE JOURNAL OF APPLIED PHYSICS
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- 巻
- 56
- 号
- 10
- 開始ページ
- 107001-1
- 終了ページ
- 107001-5
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.7567/JJAP.56.107001
- 出版者・発行元
- IOP PUBLISHING LTD
Carbon nanotubes are a novel material for next-generation applications. In this study, we generated carbon nanotube and green fluorescent protein (GFP) conjugates using affinity binding peptides. The carbon nanotube-binding motif was introduced into the N-terminus of the GFP through molecular biology methods. Multiple GFPs were successfully aligned on a single-walled carbon nanotube via the molecular recognition function of the peptide aptamer, which was confirmed through transmission electron microscopy and optical analysis. Fluorescence spectral analysis results also suggested that the carbon nanotube-GFP complex was autonomously formed with orientation and without causing protein denaturation during immobilization. This simple process has a widespread potential for fabricating carbon nanotube-biomolecule hybrid devices. (C) 2017 The Japan Society of Applied Physics
- リンク情報
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- DOI
- https://doi.org/10.7567/JJAP.56.107001
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000411924600001&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-85030629278&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0003-1838-8739
- ID情報
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- DOI : 10.7567/JJAP.56.107001
- ISSN : 0021-4922
- eISSN : 1347-4065
- ORCIDのPut Code : 66827331
- SCOPUS ID : 85030629278
- Web of Science ID : WOS:000411924600001