2017年11月
Centromere evolution and CpG methylation during vertebrate speciation
NATURE COMMUNICATIONS
- 巻
- 8
- 号
- 1
- 開始ページ
- 1833
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s41467-017-01982-7
- 出版者・発行元
- NATURE PUBLISHING GROUP
Centromeres and large-scale structural variants evolve and contribute to genome diversity during vertebrate speciation. Here, we perform de novo long-read genome assembly of three inbred medaka strains that are derived from geographically isolated subpopulations and undergo speciation. Using single-molecule real-time (SMRT) sequencing, we obtain three chromosome-mapped genomes of length similar to 734, similar to 678, and similar to 744Mbp with a resource of twenty-two centromeric regions of length 20-345kbp. Centromeres are positionally conserved among the three strains and even between four pairs of chromosomes that were duplicated by the teleost-specific whole-genome duplication 320-350 million years ago. The centromeres do not all evolve at a similar pace; rather, centromeric monomers in non-acrocentric chromosomes evolve significantly faster than those in acrocentric chromosomes. Using methylation sensitive SMRT reads, we uncover centromeres are mostly hypermethylated but have hypomethylated sub-regions that acquire unique sequence compositions independently. These findings reveal the potential of non-acrocentric centromere evolution to contribute to speciation.
- リンク情報
- ID情報
-
- DOI : 10.1038/s41467-017-01982-7
- ISSN : 2041-1723
- PubMed ID : 29184138
- Web of Science ID : WOS:000416399700006