論文

査読有り 国際誌
2020年7月16日

Functional expression of Δ12 fatty acid desaturase modulates thermoregulatory behaviour in Drosophila.

Scientific reports
  • Takuto Suito
  • ,
  • Kohjiro Nagao
  • ,
  • Kenichi Takeuchi
  • ,
  • Naoto Juni
  • ,
  • Yuji Hara
  • ,
  • Masato Umeda

10
1
開始ページ
11798
終了ページ
11798
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-020-68601-2

Polyunsaturated fatty acids (PUFAs) play crucial roles in adaptation to cold environments in a wide variety of animals and plants. However, the mechanisms by which PUFAs affect thermoregulatory behaviour remain elusive. Thus, we investigated the roles of PUFAs in thermoregulatory behaviour of Drosophila melanogaster. To this end, we generated transgenic flies expressing Caenorhabditis elegans Δ12 fatty acid desaturase (FAT-2), which converts mono-unsaturated fatty acids to PUFAs such as linoleic acid [C18:2 (n-6)] and linolenic acid [C18:3 (n-3)]. Neuron-specific expression of FAT-2 using the GAL4/UAS expression system led to increased contents of C18:2 (n-6)-containing phospholipids in central nerve system (CNS) and caused significant decreases in preferred temperature of third instar larvae. In genetic screening and calcium imaging analyses of thermoreceptor-expressing neurons, we demonstrated that ectopic expression of FAT-2 in TRPA1-expressing neurons led to decreases in preferred temperature by modulating neuronal activity. We conclude that functional expression of FAT-2 in a subset of neurons changes the thermoregulatory behaviour of D. melanogaster, likely by modulating quantities of PUFA-containing phospholipids in neuronal cell membranes.

リンク情報
DOI
https://doi.org/10.1038/s41598-020-68601-2
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32678126
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366712

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