2019年6月
Novel in vivo system to monitor tRNA expression based on the recovery of GFP fluorescence and its application for the determination of plant tRNA expression
Gene
- ,
- ,
- ,
- 巻
- 703
- 号
- 開始ページ
- 145
- 終了ページ
- 152
- DOI
- 10.1016/j.gene.2019.03.068
•Development of a novel assay system for tRNA expressed in plant cells<br />
•The assay based on recovery of the expression of GFP as a reporter<br />
•Monitoring the fluorescence of RFP for the normalization of transformation efficiency<br />
•Rapid laser-scanning microscopy of GFP or RFP fluorescence for quantitative determination<br />
•Its application to determine the expression of 17 kinds of tRNAGln genes from Arabidopsis
•The assay based on recovery of the expression of GFP as a reporter<br />
•Monitoring the fluorescence of RFP for the normalization of transformation efficiency<br />
•Rapid laser-scanning microscopy of GFP or RFP fluorescence for quantitative determination<br />
•Its application to determine the expression of 17 kinds of tRNAGln genes from Arabidopsis
- リンク情報
- ID情報
-
- DOI : 10.1016/j.gene.2019.03.068
- ISSN : 0378-1119
- PubMed ID : 30940526
- SCOPUS ID : 85064149867