論文

査読有り 筆頭著者
2022年11月12日

Establishment of a human pluripotent stem cell-derived MKX-td Tomato reporter system

Stem Cell Research & Therapy
  • Yuki Fujisawa
  • ,
  • Lu Ming
  • ,
  • Daisuke Yamada
  • ,
  • Tomoka Takao
  • ,
  • Takeshi Takarada

13
1
開始ページ
515
終了ページ
515
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1186/s13287-022-03203-5
出版者・発行元
Springer Science and Business Media LLC

Abstract

Tendon regeneration is difficult because detailed knowledge about tendon progenitor cells (TPCs), which produce tenocytes to repair tendon tissue, has not been revealed. Mohawk homeobox (MKX) is a marker of TPCs or tenocytes, but a human pluripotent stem cell (hPSC)-based reporter system that visualizes MKX+ cells has not been developed. Here, we established an hPSC-derived MKX-tdTomato reporter cell line and tested the induction ratio of MKX-tdTomato+ cells using our stepwise/xeno-free differentiation protocol. MKX-tdTomato+ cells were generated with high efficiency and expressed tendon-specific markers, including MKX, SCX, TNMD, and COL1A1. Our MKX-tdTomato hPSC line would be a useful tool for studying the development or regeneration of tendon tissue.

リンク情報
DOI
https://doi.org/10.1186/s13287-022-03203-5
URL
https://link.springer.com/content/pdf/10.1186/s13287-022-03203-5.pdf
URL
https://link.springer.com/article/10.1186/s13287-022-03203-5/fulltext.html
ID情報
  • DOI : 10.1186/s13287-022-03203-5
  • eISSN : 1757-6512

エクスポート
BibTeX RIS