論文

2021年6月28日

Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K+ channels

eLife
  • Sharon R Stevens
  • Colleen M Longley
  • Yuki Ogawa
  • Lindsay H Teliska
  • Anithachristy S Arumanayagam
  • Supna Nair
  • Juan A Oses-Prieto
  • Alma L Burlingame
  • Matthew D Cykowski
  • Mingshan Xue
  • Matthew N Rasb
  • 全て表示

10
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.7554/elife.66491
出版者・発行元
{eLife} Sciences Publications, Ltd

<jats:p>Neuronal ankyrins cluster and link membrane proteins to the actin and spectrin-based cytoskeleton. Among the three vertebrate ankyrins, little is known about neuronal Ankyrin-R (AnkR). We report AnkR is highly enriched in Pv<jats:sup>+</jats:sup> fast-spiking interneurons in mouse and human. We identify AnkR-associated protein complexes including cytoskeletal proteins, cell adhesion molecules (CAMs), and perineuronal nets (PNNs). We show that loss of AnkR from forebrain interneurons reduces and disrupts PNNs, decreases anxiety-like behaviors, and changes the intrinsic excitability and firing properties of Pv<jats:sup>+</jats:sup> fast-spiking interneurons. These changes are accompanied by a dramatic reduction in Kv3.1b K<jats:sup>+</jats:sup> channels. We identify a novel AnkR-binding motif in Kv3.1b, and show that AnkR is both necessary and sufficient for Kv3.1b membrane localization in interneurons and at nodes of Ranvier. Thus, AnkR regulates Pv<jats:sup>+</jats:sup> fast-spiking interneuron function by organizing ion channels, CAMs, and PNNs, and linking these to the underlying β1 spectrin-based cytoskeleton.</jats:p>

リンク情報
DOI
https://doi.org/10.7554/elife.66491
ID情報
  • DOI : 10.7554/elife.66491
  • ISSN : 2050-084X
  • ORCIDのPut Code : 107660018

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