論文

査読有り 国際誌
2019年6月

OsMYB108 loss-of-function enriches p-coumaroylated and tricin lignin units in rice cell walls.

The Plant journal : for cell and molecular biology
  • Takuji Miyamoto
  • ,
  • Rie Takada
  • ,
  • Yuki Tobimatsu
  • ,
  • Yuri Takeda
  • ,
  • Shiro Suzuki
  • ,
  • Masaomi Yamamura
  • ,
  • Keishi Osakabe
  • ,
  • Yuriko Osakabe
  • ,
  • Masahiro Sakamoto
  • ,
  • Toshiaki Umezawa

98
6
開始ページ
975
終了ページ
987
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/tpj.14290

Breeding approaches to enrich lignins in biomass could be beneficial to improving the biorefinery process because lignins increase biomass heating value and represent a potent source of valuable aromatic chemicals. However, despite the fact that grasses are promising lignocellulose feedstocks, limited information is yet available for molecular-breeding approaches to upregulate lignin biosynthesis in grass species. In this study, we generated lignin-enriched transgenic rice (Oryza sativa), a model grass species, via targeted mutagenesis of the transcriptional repressor OsMYB108 using CRISPR/Cas9-mediated genome editing. The OsMYB108-knockout rice mutants displayed increased expressions of lignin biosynthetic genes and enhanced lignin deposition in culm cell walls. Chemical and two-dimensional nuclear magnetic resonance (NMR) analyses revealed that the mutant cell walls were preferentially enriched in γ-p-coumaroylated and tricin lignin units, both of which are typical and unique components in grass lignins. NMR analysis also showed that the relative abundances of major lignin linkage types were altered in the OsMYB108 mutants.

リンク情報
DOI
https://doi.org/10.1111/tpj.14290
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30773774

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