論文

査読有り
2014年4月

Optogenetic Patterning of Whisker-Barrel Cortical System in Transgenic Rat Expressing Channelrhodopsin-2

PLOS ONE
  • Tatsuya Honjoh
  • ,
  • Zhi-Gang Ji
  • ,
  • Yukinobu Yokoyama
  • ,
  • Akira Sumiyoshi
  • ,
  • Yuma Shibuya
  • ,
  • Yoshiya Matsuzaka
  • ,
  • Ryuta Kawashima
  • ,
  • Hajime Mushiake
  • ,
  • Toru Ishizuka
  • ,
  • Hiromu Yawo

9
4
開始ページ
e93706
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0093706
出版者・発行元
PUBLIC LIBRARY SCIENCE

The rodent whisker-barrel system has been an ideal model for studying somatosensory representations in the cortex. However, it remains a challenge to experimentally stimulate whiskers with a given pattern under spatiotemporal precision. Recently the optogenetic manipulation of neuronal activity has made possible the analysis of the neuronal network with precise spatiotemporal resolution. Here we identified the selective expression of channelrhodopsin-2 (ChR2), an algal light-driven cation channel, in the large mechanoreceptive neurons in the trigeminal ganglion (TG) as well as their peripheral nerve endings innervating the whisker follicles of a transgenic rat. The spatiotemporal pattern of whisker irradiation thus produced a barrel-cortical response with a specific spatiotemporal pattern as evidenced by electrophysiological and functional MRI (fMRI) studies. Our methods of generating an optogenetic tactile pattern (OTP) can be expected to facilitate studies on how the spatiotemporal pattern of touch is represented in the somatosensory cortex, as Hubel and Wiesel did in the visual cortex.

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0093706
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24695456
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000334103000108&DestApp=WOS_CPL
ID情報
  • DOI : 10.1371/journal.pone.0093706
  • ISSN : 1932-6203
  • PubMed ID : 24695456
  • Web of Science ID : WOS:000334103000108

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