2020年3月1日
CD140b and CD73 are markers for human induced pluripotent stem cell-derived erythropoietin-producing cells
FEBS Open Bio
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- 巻
- 10
- 号
- 3
- 開始ページ
- 427
- 終了ページ
- 433
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/2211-5463.12800
© 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. Renal anemia in chronic kidney disease is treated with recombinant human erythropoietin (rhEPO). However, some patients with anemia do not respond well to rhEPO, emphasizing the need for a more biocompatible EPO. Differentiation protocols for hepatic lineages have been modified to enable production from human induced pluripotent stem cell (hiPSC)-derived EPO-producing cells (EPO cells). However, markers for hiPSC-EPO cells are lacking, making it difficult to purify hiPSC-EPO cells and therefore to optimize EPO production and cell counts for transplantation. To address these issues, we investigated whether CD140b and CD73 could be used as markers for hiPSC-EPO cells. We measured the expression of EPO, CD140b, and CD73 in hiPSC-EPO cells and the EPO concentration in the cell supernatant by immunohistochemistry and enzyme-linked immunosorbent assays on culture day 13, revealing that expression levels of CD140b and CD73 are correlated with the level of EPO. In addition, rates of CD140b+ CD73+ cells were observed to be correlated with the concentration of EPO. Thus, our results suggest that CD140b and CD73 may be markers for hiPSC-EPO cells.
- リンク情報
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- DOI
- https://doi.org/10.1002/2211-5463.12800
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/31977161
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7050240
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85079716834&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85079716834&origin=inward
- ID情報
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- DOI : 10.1002/2211-5463.12800
- eISSN : 2211-5463
- PubMed ID : 31977161
- PubMed Central 記事ID : PMC7050240
- SCOPUS ID : 85079716834