論文

査読有り
2017年3月

Antimicrobial susceptibility testing of rapidly growing mycobacteria isolated in Japan

BMC INFECTIOUS DISEASES
  • Shuji Hatakeyama
  • ,
  • Yuki Ohama
  • ,
  • Mitsuhiro Okazaki
  • ,
  • Yoko Nukui
  • ,
  • Kyoji Moriya

17
1
開始ページ
197
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1186/s12879-017-2298-8
出版者・発行元
BIOMED CENTRAL LTD

Background: Difficult-to-treat infections caused by rapidly growing mycobacteria (RGM) are increasingly observed in clinical settings. However, studies on antimicrobial susceptibilities and effective treatments against RGM in Japan are limited.
Methods: We conducted susceptibility testing of potential antimicrobial agents, including tigecycline and tebipenem, against RGM. Clinical RGM isolates were collected from a university hospital in Japan between December 2010 and August 2013. They were identified using matrix-assisted laser desorption/ionization time-offlight mass spectrometry and the sequencing of 16S rRNA, rpoB, and hsp65 genes. The samples were utilized for susceptibility testing using 16 antimicrobials, with frozen broth microdilution panels.
Results: Forty-two isolates were obtained: 13, Mycobacterium abscessus complex; 12, Mycobacterium chelonae; 9, Mycobacterium fortuitum; and 8, M. fortuitum group species other than M. fortuitum. Different antimicrobial susceptibility patterns were observed between RGM species. Clarithromycin-susceptible strain rates were determined to be 0, 62, and 100% for M. fortuitum, M. abscessus complex, and M. chelonae, respectively. M. abscessus complex (100%) and > 80% M. chelonae isolates were non-susceptible, while 100% M. fortuitum group isolates were susceptible to moxifloxacin. Linezolid showed good activity against 77% M. abscessus complex, 89% M. fortuitum, and 100% M. chelonae isolates. Regardless of species, all tested isolates were inhibited by tigecycline at very low minimal inhibitory concentrations (MICs) of = 0.5 mu g/mL. MICs of tebipenem, an oral carbapenem, were <= 4 mu g/mL against all M. fortuitum group isolates.
Conclusions: Our study demonstrates the importance of correct identification and antimicrobial susceptibility testing, including the testing of potential new agents, in the management of RGM infections.

リンク情報
DOI
https://doi.org/10.1186/s12879-017-2298-8
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28270102
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000397392600005&DestApp=WOS_CPL
ID情報
  • DOI : 10.1186/s12879-017-2298-8
  • ISSN : 1471-2334
  • PubMed ID : 28270102
  • Web of Science ID : WOS:000397392600005

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