論文

査読有り
2017年6月1日

Selective Manipulation of Discrete Mannosidase Activities in the Endoplasmic Reticulum by Using Reciprocally Selective Inhibitors

ChemBioChem
  • Taiki Kuribara
  • ,
  • Makoto Hirano
  • ,
  • Gaetano Speciale
  • ,
  • Spencer J. Williams
  • ,
  • Yukishige Ito
  • ,
  • Kiichiro Totani

18
11
開始ページ
1027
終了ページ
1035
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/cbic.201700081
出版者・発行元
Wiley-VCH Verlag

Within the endoplasmic reticulum, immature glycoproteins are sorted into secretion and degradation pathways through the sequential trimming of mannose residues from Man9GlcNAc2 to Man5GlcNAc2 by the combined actions of assorted α-1,2-mannosidases. It has been speculated that specific glycoforms encode signals for secretion and degradation. However, it is unclear whether the specific signal glycoforms are produced by random mannosidase action or are produced regioselectively in a sequenced manner by specific α-1,2-mannosidases. Here, we report the identification of a set of selective mannosidase inhibitors and development of conditions for their use that enable production of distinct pools of Man8GlcNAc2 isomers from a structurally defined synthetic Man9GlcNAc2 substrate in an endoplasmic reticulum fraction. Glycan processing analysis with these inhibitors provides the first biochemical evidence for selective production of the signal glycoforms contributing to traffic control in glycoprotein quality control.

リンク情報
DOI
https://doi.org/10.1002/cbic.201700081
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28371030
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000402966900011&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/cbic.201700081
  • ISSN : 1439-7633
  • ISSN : 1439-4227
  • ORCIDのPut Code : 34890704
  • PubMed ID : 28371030
  • SCOPUS ID : 85018755713
  • Web of Science ID : WOS:000402966900011

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