論文

査読有り
2020年

Rapid differentiation of human dental pulp stem cells to neuron-like cells by high K+ stimulation.

Biophysics and physicobiology
  • Yuki Kogo
  • ,
  • Chiaki Seto
  • ,
  • Yuki Totani
  • ,
  • Mai Mochizuki
  • ,
  • Taka Nakahara
  • ,
  • Kotaro Oka
  • ,
  • Tohru Yoshioka
  • ,
  • Etsuro Ito

17
開始ページ
132
終了ページ
139
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.2142/biophysico.BSJ-2020023

As human-origin cells, human dental pulp stem cells (hDPSCs) are thought to be potentially useful for biological and medical experiments. They are easily obtained from lost primary teeth or extracted wisdom teeth, and they are mesenchymal stem cells that are known to differentiate into osteoblasts, chondrocytes, and adipocytes. Although hDPSCs originate from neural crest cells, it is difficult to induce hDPSCs to differentiate into neuron-like cells. To facilitate their differentiation into neuron-like cells, we evaluated various differentiation conditions. Activation of K+ channels is thought to regulate the intracellular Ca2+ concentration, allowing for manipulation of the cell cycle to induce the differentiation of hDPSCs. Therefore, in addition to a conventional neural cell differentiation protocol, we activated K+ channels in hDPSCs. Immunocyto-chemistry and real-time PCR revealed that applying a combination of 3 stimuli (high K+ solution, epigenetic reprogramming solution, and neural differentiation solution) to hDPSCs increased their expression of neuronal markers, such as β3-tubulin, postsynaptic density protein 95, and nestin within 5 days, which led to their rapid differentiation into neuron-like cells. Our findings indicate that epigenetic reprogramming along with cell cycle regulation by stimulation with high K+ accelerated the differentiation of hDPSCs into neuron-like cells. Therefore, hDPSCs can be used in various ways as neuron-like cells by manipulating their cell cycle.

リンク情報
DOI
https://doi.org/10.2142/biophysico.BSJ-2020023
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33240740
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7671740
ID情報
  • DOI : 10.2142/biophysico.BSJ-2020023
  • PubMed ID : 33240740
  • PubMed Central 記事ID : PMC7671740

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