The demand for entrepreneurship education has been increasing in many countries. Higher education programs in entrepreneurship are important to increase entrepreneurial thinking. The effectiveness of these educational programs is of interest to researchers, even though some acknowledge difficulties in evaluating the efficacy of these programs (McMullan and Gillin, 2001; Cox et al, 2002). The United States and Europe lead global interest in determining entrepreneurship education outcomes. However, little is known about the effects of entrepreneurship education in other regions, including Japan. Inada (2018b) reports that only 7 out of 23 business schools in Japan include entrepreneurship courses, and research on the effects of these higher education courses is scant. The purpose of this study was to investigate changes in students' entrepreneurial competencies before and after participating in a higher education course in Japan dedicated to entrepreneurship. Participants were 21 MBA students who attended an entrepreneurship course at a Japanese business school. During the course students created a business plan in English. Survey data were collected from June-July 2017, after the 7-week course on entrepreneurship. The questionnaire survey was based on the three main elements of entrepreneurial competencies identified by Lackéus (2015): knowledge, skills, and attitudes. Results revealed statistically significant differences in knowledge and attitudes before and after entrepreneurship education. In the knowledge domain, self-insight, which includes knowledge regarding personal fit with being an entrepreneur or being entrepreneurial, was improved. Regarding attitudes, entrepreneurial passion, self-efficacy, and entrepreneurial identity were improved. Although the difference in skills fell short of statistical significance, improvement in this area was supported by a medium to large effect size. Considering the effect sizes, skills, mental models in knowledge, interpersonal skills, uncertainty/ ambiguity tolerance, and perseverance in attitudes all improved following the entrepreneurship course based on creating a business plan. As indicated by Nabi et al (2017), it is important to accumulate the effects of entrepreneurship education and develop it for future research.