論文

国際誌
2020年3月

Clinical usefulness of anti-M-type phospholipase-A-receptor antibodies in patients with membranous nephropathy and the comparison of three quantification methods.

Immunological medicine
  • Yasuhiro Katsumata
  • ,
  • Yuko Okamoto
  • ,
  • Takahito Moriyama
  • ,
  • Rina Moriyama
  • ,
  • Manabu Kawamoto
  • ,
  • Masanori Hanaoka
  • ,
  • Keiko Uchida
  • ,
  • Kosaku Nitta
  • ,
  • Masayoshi Harigai

43
1
開始ページ
47
終了ページ
56
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/25785826.2019.1710079

Associations between anti-M-type phospholipase A2 receptor (PLA2R) antibodies and disease activity and prognosis have been suggested in primary membranous nephropathy (MN); however, more evidence is needed. We aimed to establish a clinically useful method to measure anti-PLA2R antibodies. We developed a western blot assay and a cell-based enzyme-linked immunosorbent assay (ELISA). Anti-PLA2R antibodies were evaluated retrospectively using these assays and the commercial solid-phase ELISA. Anti-PLA2R antibodies were detected in 12, 6, and 12 out of 23 Japanese patients with biopsy-proven primary MN using the western blot, the cell-based ELISA, and the solid-phase ELISA, respectively. The samples of the lupus MN patients tested negative. The levels of proteinuria correlated moderately with the titres of anti-PLA2R antibodies measured by the three methods (r = 0.39-0.47). Anti-PLA2R antibodies were significantly associated with physicians' decisions on immunosuppressive treatment without prior knowledge of anti-PLA2R antibody positivity (p < .01). In the longitudinal analysis, the titres of anti-PLA2R antibodies measured by the solid-phase ELISA declined significantly following treatment (p = .03). In conclusion, these results suggest the usefulness of anti-PLA2R antibody as a diagnostic, prognostic, and surrogate biomarker in primary MN. The three methods proved to be reliable for measuring anti-PLA2R antibody titres, but their performances differ.

リンク情報
DOI
https://doi.org/10.1080/25785826.2019.1710079
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31910103
ID情報
  • DOI : 10.1080/25785826.2019.1710079
  • PubMed ID : 31910103

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