MISC

国際誌
2017年

Analyzing pERK Activation During Planarian Regeneration.

Methods in molecular biology (Clifton, N.J.)
  • Susanna Fraguas
  • ,
  • Yoshihiko Umesono
  • ,
  • Kiyokazu Agata
  • ,
  • Francesc Cebrià

1487
開始ページ
303
終了ページ
315
記述言語
英語
掲載種別
Scientific Journal

Planarians are an ideal model in which to study stem cell-based regeneration. After amputation, planarian pluripotent stem cells surrounding the wound proliferate to produce the regenerative blastema, in which they differentiate into the missing tissues and structures. Recent independent studies in planarians have shown that Smed-egfr-3, a gene encoding a homologue of epidermal growth factor (EGF) receptors, and DjerkA, which encodes an extracellular signal-regulated kinase (ERK), may control cell differentiation and blastema growth. However, because these studies were carried in two different planarian species, the relationship between these two genes remains unclear. We have optimized anti-pERK immunostaining in Schmidtea mediterranea using the original protocol developed in Dugesia japonica. Both protocols are reported here as most laboratories worldwide work with one of these two species. Using this protocol we have determined that Smed-egfr-3 appears to be necessary for pERK activation during planarian regeneration.

リンク情報
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27924577

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