論文

国際誌
2019年7月29日

In vivo direct reprogramming of glial linage to mature neurons after cerebral ischemia.

Scientific reports
  • Toru Yamashita
  • ,
  • Jingwei Shang
  • ,
  • Yumiko Nakano
  • ,
  • Ryuta Morihara
  • ,
  • Kota Sato
  • ,
  • Mami Takemoto
  • ,
  • Nozomi Hishikawa
  • ,
  • Yasuyuki Ohta
  • ,
  • Koji Abe

9
1
開始ページ
10956
終了ページ
10956
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-019-47482-0

The therapeutic effect of in vivo direct reprogramming on ischemic stroke has not been evaluated. In the present study, a retroviral solution (1.5-2.0 × 107 /ul) of mock pMX-GFP (n = 13) or pMX-Ascl1/Sox2/NeuroD1 (ASN) (n = 14) was directly injected into the ipsilateral striatum and cortex 3 days after 30 min of transient cerebral ischemia. The reprogrammed cells first expressed neuronal progenitor marker Dcx 7 and 21 days after viral injection, then expressed mature neuronal marker NeuN. This was accompanied by morphological changes, including long processes and synapse-like structures, 49 days after viral injection. Meanwhile, therapeutic improvement was not detected both in clinical scores or infarct volume. The present study provides a future novel self-repair strategy for ischemic stroke with beneficial modifications of the inducer-suppressor balance.

リンク情報
DOI
https://doi.org/10.1038/s41598-019-47482-0
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31358888
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662847
ID情報
  • DOI : 10.1038/s41598-019-47482-0
  • PubMed ID : 31358888
  • PubMed Central 記事ID : PMC6662847

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