2019年7月29日
In vivo direct reprogramming of glial linage to mature neurons after cerebral ischemia.
Scientific reports
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- 巻
- 9
- 号
- 1
- 開始ページ
- 10956
- 終了ページ
- 10956
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s41598-019-47482-0
The therapeutic effect of in vivo direct reprogramming on ischemic stroke has not been evaluated. In the present study, a retroviral solution (1.5-2.0 × 107 /ul) of mock pMX-GFP (n = 13) or pMX-Ascl1/Sox2/NeuroD1 (ASN) (n = 14) was directly injected into the ipsilateral striatum and cortex 3 days after 30 min of transient cerebral ischemia. The reprogrammed cells first expressed neuronal progenitor marker Dcx 7 and 21 days after viral injection, then expressed mature neuronal marker NeuN. This was accompanied by morphological changes, including long processes and synapse-like structures, 49 days after viral injection. Meanwhile, therapeutic improvement was not detected both in clinical scores or infarct volume. The present study provides a future novel self-repair strategy for ischemic stroke with beneficial modifications of the inducer-suppressor balance.
- リンク情報
- ID情報
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- DOI : 10.1038/s41598-019-47482-0
- PubMed ID : 31358888
- PubMed Central 記事ID : PMC6662847