2012年7月
Slantingly cross loading sample system enables simultaneous performance of separation and mixture to detect molecular interactions on thin-layer chromatography
JOURNAL OF CHROMATOGRAPHY A
- ,
- ,
- ,
- 巻
- 1245
- 号
- 開始ページ
- 183
- 終了ページ
- 189
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.chroma.2012.05.011
- 出版者・発行元
- ELSEVIER SCIENCE BV
Anthocyanins are major flower pigments that can be affected by copigments, colorless compounds that can modify anthocyanin coloration to more intense and bluer. Thin-layer chromatography (TLC) is an available technique to separate and analyze anthocyanins and copigments. To easily and comprehensively detect copigments, we added function of mixture of compounds to TLC; by slantingly cross loading samples on TLC, compounds are symmetrically developed at various angle lines from the upper origin to individual R-f values and cross each other in an orderly fashion, where mixture is simultaneously performed with separation. Occurrence of copigments can be detected as a coloration change on the developed line of anthocyanin. Pink sweet pea (Lathyrus odoratus L) petals were analyzed by the cross-TLC and a more intense spot and a paler spot on the anthocyanin line were detected. As each spot overlapped with an ultraviolet absorbance line, each of these ultraviolet absorption compounds was purified and identified as kaempferol 3-rhamnoside and 2-cyanoethyl-isoxazolin-5-one, respectively. Whereas kaempferol 3-rhamnoside is a flavonoid and had a general copigment effect of more intense and bluer coloration change, 2-cyanoethyl-isoxazolin-5-one is a compound whose structure is outside of conventional categories of copigments and had a novel effect to change anthocyanin coloration paler while maintaining color tone. We determined that the search for copigments should be carried out without pre-existing prediction of structures and effects. We have shown that slantingly cross loading samples system on plate-type chromatography is an effective technique for such comprehensive analysis of molecular interaction. Crown Copyright (c) 2012 Published by Elsevier B.V. All rights reserved.
- リンク情報
-
- DOI
- https://doi.org/10.1016/j.chroma.2012.05.011
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201202224019785559
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/22640837
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000305846500023&DestApp=WOS_CPL
- URL
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84861978884&origin=inward
- ID情報
-
- DOI : 10.1016/j.chroma.2012.05.011
- ISSN : 0021-9673
- J-Global ID : 201202224019785559
- PubMed ID : 22640837
- SCOPUS ID : 84861978884
- Web of Science ID : WOS:000305846500023