論文

査読有り
2016年

Platelets attenuate production of cytokines and nitric oxide by macrophages in response to bacterial endotoxin

PLATELETS
  • Yusuke Ando
  • ,
  • Teruaki Oku
  • ,
  • Tsutomu Tsuji

27
4
開始ページ
344
終了ページ
350
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3109/09537104.2015.1103369
出版者・発行元
TAYLOR & FRANCIS INC

Considerable evidence has been accumulated concerning the roles of platelets in immune responses. In the present study, we examined the functional modulation of macrophages by platelets. When mouse bone marrow-derived macrophages (BMDMs) were co-cultured with platelets, BMDMs produced lower levels of nitric oxide (NO), tumor necrosis factor-alpha (TNF)-alpha, and interleukin (IL)-6 in response to a bacterial endotoxin (LPS) and zymosan. The attenuation in the macrophage susceptibility to LPS appeared to be mediated by soluble factors secreted from platelets. The mRNA levels of NOS2 (iNOS), TNF-alpha, and IL-6 in LPS-stimulated BMDMs that had been cultured with a conditioned medium of platelets were also decreased as analyzed by RT-qPCR. The ability of the platelet-conditioned medium to suppress macrophage NO production was recovered in a high-molecular-weight fraction (>670 kDa) after gel-filtration chromatography on a Superose 6 column. These results suggest that platelets control the susceptibility of macrophages to prevent excessive responses to LPS and provide mechanistic insight into a previous report that experimental thrombocytopenia aggravated organ failure in LPS-induced endotoxemia.

Web of Science ® 被引用回数 : 18

リンク情報
DOI
https://doi.org/10.3109/09537104.2015.1103369
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26588084
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000377104000011&DestApp=WOS_CPL
ID情報
  • DOI : 10.3109/09537104.2015.1103369
  • ISSN : 0953-7104
  • eISSN : 1369-1635
  • PubMed ID : 26588084
  • Web of Science ID : WOS:000377104000011

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