MISC

2008年5月29日

Cdc7 kinase mediates Claspin phosphorylation in DNA replication checkpoint

Oncogene
  • J. M. Kim
  • ,
  • N. Kakusho
  • ,
  • M. Yamada
  • ,
  • Y. Kanoh
  • ,
  • N. Takemoto
  • ,
  • H. Masai

27
24
開始ページ
3475
終了ページ
3482
記述言語
英語
掲載種別
DOI
10.1038/sj.onc.1210994

Cdc7 kinase is evolutionarily conserved and is involved in initiation and progression of DNA replication. However, roles of Cdc7 in checkpoint responses remain largely unknown. In this study, we show that deletion of the Cdc7 genes in mouse embryonic stem (ES) cells abrogates hydroxyurea (HU)- or UV-induced activation of Chk1. HU-induced Chk1 activation is also impaired in human cancer cell lines in which Cdc7 is depleted by siRNA, and Cdc7-depleted cells are more sensitive to HU treatment. In contrast, ATR and Rad17 are relocated to chromatin in these cells following HU treatment, indicating that stalled DNA replication forks are detected normally. Cdc7-depleted cells exhibit defects in chromatin association and phosphorylation of Claspin, suggesting that Cdc7 exerts its effect at least partially through Claspin. Consistent with this prediction, Cdc7 interacts with and phosphorylates Claspin. We propose that Cdc7 is required for activation of the ATR-Chk1 checkpoint pathway through regulation of Claspin. © 2008 Nature Publishing Group All rights reserved.

リンク情報
DOI
https://doi.org/10.1038/sj.onc.1210994
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18084324
ID情報
  • DOI : 10.1038/sj.onc.1210994
  • ISSN : 0950-9232
  • ISSN : 1476-5594
  • PubMed ID : 18084324
  • SCOPUS ID : 44449112225

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