論文

査読有り
2005年12月

Overexpression of drebrin A in immature neurons induces the accumulation of F-actin and PSD-95 into dendritic filopodia, and the formation of large abnormal protrusions

MOLECULAR AND CELLULAR NEUROSCIENCE
  • T Mizui
  • ,
  • H Takahashi
  • ,
  • Y Sekino
  • ,
  • T Shirao

30
4
開始ページ
630
終了ページ
638
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.mcn.2005.06.013
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Drebrin A is a neuron-specific F-actin binding protein, and plays a pivotal role in the spine formation. In this study, we expressed drebrin A tagged with green fluorescent protein (GFP-DA) in hippocampal neurons at 7-9 days in vitro when presynaptic terminals are not fully maturated. GFP-DA was accumulated in dendritic protrusions and formed large abnormal structures. Since these structures were similar to filopodia in terms of lack of MAP2 immunostaining, we named them "megapodia" meaning large dendritic filopodia. F-actin and PSD-95 were also accumulated in megapodia, and their amounts were significantly correlated with that of GFP-DA. However, the expression of GFP-DA did not result in the promotion of the morphological change from filopodia into spines. These results demonstrate that drebrin A accumulates spine-resident proteins via protein-protein interaction in filopodia, and suggest that the spine formation requires the concurrence of the increase of drebrin-A expression and the functional presynaptic contact. (c) 2005 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.mcn.2005.06.013
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/16456930
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000234637700022&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.mcn.2005.06.013
  • ISSN : 1044-7431
  • PubMed ID : 16456930
  • Web of Science ID : WOS:000234637700022

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