論文

査読有り 筆頭著者 国際誌
2011年10月

A non-destructive screenable marker, OsFAST, for identifying transgenic rice seeds.

Plant signaling & behavior
  • Takashi Shimada
  • ,
  • Yoichi Ogawa
  • ,
  • Tomoo Shimada
  • ,
  • Ikuko Hara-Nishimura

6
10
開始ページ
1454
終了ページ
6
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.4161/psb.6.10.17344

The production of transgenic plants has contributed greatly to plant research. Previously, an improved method for screening transgenic Arabidopsis thaliana seeds using the FAST (Fluorescence-Accumulating-Seed Technology) method and FAST marker was reported. Arabidopsis seeds containing the FAST marker may be visually screened using a fluorescence stereomicroscope or blue LED handy-type instrument. Although the FAST method was originally designed for Arabidopsis screens, this study endeavors to adapt this method for the screening of other plants. Here, an optimized technology, designated the OsFAST method, is presented as a useful tool for screening transgenic rice seeds. The OsFAST method is based on the expression of the OsFAST-G marker under the control of a seed-embryo-specific promoter, similar to the Arabidopsis FAST-G marker. The OsFAST method provides a simple and non-destructive method for identifying transgenic rice seeds. It is proposed that the FAST method is adaptable to various plant species and will enable a deeper analysis of the floral-dip method.

リンク情報
DOI
https://doi.org/10.4161/psb.6.10.17344
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21904112
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3256369
ID情報
  • DOI : 10.4161/psb.6.10.17344
  • ISSN : 1559-2316
  • PubMed ID : 21904112
  • PubMed Central 記事ID : PMC3256369

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