論文

査読有り 筆頭著者 国際誌
2019年7月1日

Enrichment of Phosphatidylinositol 4,5-Bisphosphate in the Extra-Invasive Hyphal Membrane Promotes Colletotrichum Infection of Arabidopsis thaliana.

Plant & cell physiology
  • Takashi L Shimada
  • ,
  • Shigeyuki Betsuyaku
  • ,
  • Noriko Inada
  • ,
  • Kazuo Ebine
  • ,
  • Masaru Fujimoto
  • ,
  • Tomohiro Uemura
  • ,
  • Yoshitaka Takano
  • ,
  • Hiroo Fukuda
  • ,
  • Akihiko Nakano
  • ,
  • Takashi Ueda

60
7
開始ページ
1514
終了ページ
1524
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/pcp/pcz058

Pathogenic fungi from the genus Colletotrichum form invasive hyphae; the hyphae are surrounded by an extra-invasive hyphal membrane (EIHM), which is continuous with the plant plasma membrane. Although the EIHM plays a crucial role as the interface between plant and fungal cells, its precise function during Colletotrichum infection remains elusive. Here, we show that enrichment of phosphoinositides (PIs) has a crucial role in Colletotrichum infection. We observed the localization of PIs in Arabidopsis thaliana cells infected by A. thaliana-adapted Colletotrichum higginsianum (Ch), and found that phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] was extremely enriched in the EIHM during Ch infection. We also found that phosphatidylinositol 4-phosphate-5 kinase (PIP5K), which catalyzes production of PI(4,5)P2, also accumulated at the EIHM. The overexpression of PIP5K3 in A. thaliana increased hyphal invasion by Ch. An exocytic factor, EXO84b, was targeted to the EIHM during Ch infection, although endocytic factors such as CLATHRIN LIGHT CHAIN 2 and FLOTILLIN 1 did not. Intriguingly, the interfacial membranes between A. thaliana and powdery mildew- or downy mildew-causing pathogens did not accumulate PI(4,5)P2. These results suggest that Ch could modify the PI(4,5)P2 levels in the EIHM to increase the exocytic membrane/protein supply of the EIHM for successful infection. Our results also suggest that PI(4,5)P2 biosynthesis is a promising target for improved defense against Colletotrichum infection.

リンク情報
DOI
https://doi.org/10.1093/pcp/pcz058
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30989198
ID情報
  • DOI : 10.1093/pcp/pcz058
  • ISSN : 0032-0781
  • PubMed ID : 30989198

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