論文

2023年10月31日

Determination of the factors responsible for the tropism of SARS-CoV-2-related bat coronaviruses to Rhinolophus bat ACE2

Journal of Virology
  • Shigeru Fujita
  • Yusuke Kosugi
  • Izumi Kimura
  • Kenzo Tokunaga
  • Jumpei Ito
  • Kei Sato
  • Keita Matsuno
  • Naganori Nao
  • Hirofumi Sawa
  • Shinya Tanaka
  • Masumi Tsuda
  • Lei Wang
  • Yoshikata Oda
  • Zannatul Ferdous
  • Kenji Shishido
  • Takasuke Fukuhara
  • Tomokazu Tamura
  • Rigel Suzuki
  • Saori Suzuki
  • Hayato Ito
  • Yu Kaku
  • Naoko Misawa
  • Arnon Plianchaisuk
  • Ziyi Guo
  • Alfredo A. Hinay
  • Keiya Uriu
  • Jarel Elgin M. Tolentino
  • Luo Chen
  • Lin Pan
  • Mai Suganami
  • Mika Chiba
  • Ryo Yoshimura
  • Kyoko Yasuda
  • Keiko Iida
  • Naomi Ohsumi
  • Adam P. Strange
  • Shiho Tanaka
  • Kazuhisa Yoshimura
  • Kenji Sadamasu
  • Mami Nagashima
  • Hiroyuki Asakura
  • Isao Yoshida
  • So Nakagawa
  • Akifumi Takaori-Kondo
  • Kayoko Nagata
  • Ryosuke Nomura
  • Yoshihito Horisawa
  • Yusuke Tashiro
  • Yugo Kawai
  • Kazuo Takayama
  • Rina Hashimoto
  • Sayaka Deguchi
  • Yukio Watanabe
  • Ayaka Sakamoto
  • Naoko Yasuhara
  • Takao Hashiguchi
  • Tateki Suzuki
  • Kanako Kimura
  • Jiei Sasaki
  • Yukari Nakajima
  • Hisano Yajima
  • Takashi Irie
  • Ryoko Kawabata
  • Kaori Tabata
  • Terumasa Ikeda
  • Hesham Nasser
  • Ryo Shimizu
  • M. S. T. Monira Begum
  • Michael Jonathan
  • Yuka Mugita
  • Otowa Takahashi
  • Kimiko Ichihara
  • Chihiro Motozono
  • Takamasa Ueno
  • Mako Toyoda
  • Akatsuki Saito
  • Maya Shofa
  • Yuki Shibatani
  • Tomoko Nishiuchi
  • Kotaro Shirakawa
  • 全て表示

97
10
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1128/jvi.00990-23
出版者・発行元
American Society for Microbiology

ABSTRACT

Differences in host angiotensin converting enzyme 2 (ACE2) genes may affect the host range of SARS-CoV-2-related coronaviruses (SC2r-CoVs) and further determine the tropism of host ACE2 for the infection receptor. However, the factor(s) responsible for determining the host tropism of SC2r-CoVs, which may in part be determined by the tropism of host ACE2 usage, remains unclear. Here, we use the pseudoviruses with the spike proteins of two Laotian SC2r-CoVs, BANAL-20-236 and BANAL-20-52, and the cells expressing ACE2 proteins of eight different Rhinolophus bat species to show that these two spikes have different tropisms for Rhinolophus bat ACE2. Through structural analysis and cell culture experiments, we demonstrate that this tropism is determined by residue 493 of the spike and residues 31 and 35 of ACE2. Our results suggest that SC2r-CoVs exhibit differential ACE2 tropism, which may be driven by adaptation to different Rhinolophus bat ACE2 proteins.

IMPORTANCE

The efficiency of infection receptor use is the first step in determining the species tropism of viruses. After the coronavirus disease 2019 pandemic, a number of SARS-CoV-2-related coronaviruses (SC2r-CoVs) were identified in Rhinolophus bats, and some of them can use human angiotensin converting enzyme 2 (ACE2) for the infection receptor without acquiring additional mutations. This means that the potential of certain SC2r-CoVs to cause spillover from bats to humans is "off-the-shelf." However, both SC2r-CoVs and Rhinolophus bat species are highly diversified, and the host tropism of SC2r-CoVs remains unclear. Here, we focus on two Laotian SC2r-CoVs, BANAL-20-236 and BANAL-20-52, and determine how the tropism of SC2r-CoVs to Rhinolophus bat ACE2 is determined at the amino acid resolution level.

リンク情報
DOI
https://doi.org/10.1128/jvi.00990-23
URL
https://journals.asm.org/doi/pdf/10.1128/jvi.00990-23
ID情報
  • DOI : 10.1128/jvi.00990-23
  • ISSN : 0022-538X
  • eISSN : 1098-5514

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