論文

査読有り
2017年1月

IL-22/IL-22R1 signaling regulates the pathophysiology of chronic rhinosinusitis with nasal polyps via alteration of MUC1 expression

ALLERGOLOGY INTERNATIONAL
  • Yasuyuki Noyama
  • ,
  • Mitsuhiro Okano
  • ,
  • Tazuko Fujiwara
  • ,
  • Shin Kariya
  • ,
  • Takaya Higaki
  • ,
  • Takenori Haruna
  • ,
  • Sei-ichiro Makihara
  • ,
  • Kengo Kanai
  • ,
  • Takahisa Koyama
  • ,
  • Masami Taniguchi
  • ,
  • Jun-ichi Ishitoya
  • ,
  • Akira Kanda
  • ,
  • Yoshiki Kobayashi
  • ,
  • Mikiya Asako
  • ,
  • Koichi Tomoda
  • ,
  • Kazunori Nishizaki

66
1
開始ページ
42
終了ページ
51
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.alit.2016.04.017
出版者・発行元
JAPANESE SOCIETY ALLERGOLOGY

Background: IL-22 is an IL-10-family cytokine that regulates chronic inflammation. We investigated the role of IL-22 and its receptor, IL-22R1, in the pathophysiology of chronic rhinosinusitis with nasal polyps (CRSwNP).
Methods: IL-22 and IL-22R1 protein and mRNA expression in NP and in uncinate tissues (UT) from CRS and non-CRS patients was examined using immunohistochemistry and real-time PCR, respectively. Dispersed NP and UT cells were cultured with the Staphylococcus aureus exotoxins, staphylococcal enterotoxin B and alpha-toxin, following which exotoxin-induced IL-22 levels and their association with clinicopathological factors were analyzed. Effects of IL-22 on MUC1 expression and cytokine release in NP cells were also determined.
Results: IL-22 and IL-22R1 in NP were mainly expressed in infiltrating inflammatory cells and in epithelial cells, respectively. IL-22 mRNA levels in NP were significantly higher than those in UTs from non-CRS patients whereas IL-22R1 levels were conversely lower in NPs. NP cells produced substantial amounts of IL -22 in response to exotoxins. Exotoxin-induced IL -22 production by NP cells significantly and negatively correlated with the degree of local eosinophilia and postoperative computed tomography (CT) score, whereas conversely it positively correlated with the forced expiratory volume in 1s (FEV1)/forced vital capacity (FVC) ratio. IL -22 significantly enhanced MUC1 mRNA expression in NP cells. IL-22-induced MUC1 mRNA levels were significantly and positively correlated with IL-22R1 mRNA levels in NPs.
Conclusions: These data suggest that imbalance of IL-22/IL-22R1 signaling regulates the pathogenesis of CRSwNP, including local eosinophilia, via alteration of MUC1 expression. Copyright (C) 2016, Japanese Society of Allergology. Production and hosting by Elsevier B.V.

Web of Science ® 被引用回数 : 8

リンク情報
DOI
https://doi.org/10.1016/j.alit.2016.04.017
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27502468
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000393735600008&DestApp=WOS_CPL