2020年12月
Protocol for Disome Profiling to Survey Ribosome Collision in Humans and Zebrafish
STAR Protocols
- ,
- ,
- 巻
- 1
- 号
- 3
- 開始ページ
- 100168
- 終了ページ
- 100168
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.xpro.2020.100168
- 出版者・発行元
- Elsevier BV
Ribosomes often encounter obstacles during translation elongation and thus collide with each other. Disome profiling, an optimized ribosome profiling method, specifically sequences the long ribosome footprints generated from collided ribosomes produced by the ribosome pause and thus allows the survey of sites in a genome-wide manner. This protocol details the procedure from lysate preparation of human tissue cultures and zebrafish embryos to sequencing library construction. For complete details on the use and execution of this protocol, please refer to Han et al. (2020).
- リンク情報
- ID情報
-
- DOI : 10.1016/j.xpro.2020.100168
- ISSN : 2666-1667
- PubMed ID : 33377062
- PubMed Central 記事ID : PMC7757362