Kouji Nakamura - My portal

Profile Information

Affiliation: Professor, Faculty of Life and Environmental Sciences, University of Tsukuba

Contact information: nakamura.koujibiol.tsukuba.ac.jp
J-GLOBAL ID: 200901070442598637
researchmap Member ID: 1000312778

Research Areas

Life sciences / Genetics /

Research History

Feb, 2011 - Present

Graduate school of Life and environmental sciences, University of Tsukuba
Apr, 2007 - Jan, 2011

准教授, 大学院生命環境科学研究科, 筑波大学
Apr, 2004 - Mar, 2007

助教授, 大学院生命環境科学研究科, 筑波大学
May, 2002 - Mar, 2004

Institute of Biological Sciences, University of Tsukuba
Jun, 1992 - Apr, 2002

Institute of Biological Sciences, University of Tsukuba
Jul, 1990 - May, 1992

Institute of Biological Sciences, University of Tsukuba
Apr, 1989 - Jun, 1990

特別研究員, 日本学術振興会

Education

- 1989

Graduate School, Division of Bioresearch, University of Tsukuba

Awards

2002

日本農芸化学奨励賞

中村幸治

Papers

Temperature-regulated heterogeneous extracellular matrix gene expression defines biofilm morphology inClostridium perfringens

Obana, Nozomu, Nakamura, Kouji, Nomura, Nobuhiko

NPJ BIOFILMS AND MICROBIOMES, 6(1), Jul, 2020
Super-Agrobacterium ver. 4: Improving the Transformation Frequencies and Genetic Engineering Possibilities for Crop Plants

Nonaka, Satoko, Someya, Tatsuhiko, Kadota, Yasuhiro, Nakamura, Kouji, Ezura, Hiroshi

Frontiers in plant science, 10 1204-1204, Oct, 2019
Immunoactive clostridial membrane vesicle production is regulated by a sporulation factor

Nozomu Obana, Ryoma Nakao, Kyoko Nagayama, Kouji Nakamura, Hidenobu Senpuku, Nobuhiko Nomura

Infection and Immunity, 85(5), May 1, 2017 Peer-reviewed
A Sporulation Factor Is Involved in the Morphological Change of Clostridium perfringens Biofilms in Response to Temperature

Nozomu Obana, Kouji Nakamura, Nobuhiko Nomura

JOURNAL OF BACTERIOLOGY, 196(8) 1540-1550, Apr, 2014
SP10 Infectivity Is Aborted after Bacteriophage SP10 Infection Induces nonA Transcription on the Prophage SP beta Region of the Bacillus subtilis Genome

Tatsuya Yamamoto, Nozomu Obana, Lii Mien Yee, Kei Asai, Nobuhiko Nomura, Kouji Nakamura

JOURNAL OF BACTERIOLOGY, 196(3) 693-706, Feb, 2014 Peer-reviewed
Structural Requirement in Clostridium perfringens Collagenase mRNA 5 ' Leader Sequence for Translational Induction through Small RNA-mRNA Base Pairing

Nozomu Obana, Nobuhiko Nomura, Kouji Nakamura

JOURNAL OF BACTERIOLOGY, 195(12) 2937-2946, Jun, 2013 Peer-reviewed
Morphological change of biofilm in response to temperature in Clostridium perfringens

Obana,Nozomu, Nakamura,Kouji, Nomura,Nobuhiko

日本生物工学会大会講演要旨集, 64 54-54, Sep, 2012
Crystal structure of Hfq from Bacillus subtilis in complex with SELEX-derived RNA aptamer: insight into RNA-binding properties of bacterial Hfq

Tatsuhiko Someya, Seiki Baba, Mai Fujimoto, Gota Kawai, Takashi Kumasaka, Kouji Nakamura

NUCLEIC ACIDS RESEARCH, 40(4) 1856-1867, Feb, 2012 Peer-reviewed
A Novel Toxin Regulator, the CPE1446-CPE1447 Protein Heteromeric Complex, Controls Toxin Genes in Clostridium perfringens

Nozomu Obana, Kouji Nakamura

JOURNAL OF BACTERIOLOGY, 193(17) 4417-4424, Sep, 2011 Peer-reviewed
Secretion of biologically-active human interferon-beta by Bacillus subtilis

Hiroshi Kakeshita, Yasushi Kageyama, Keiji Endo, Masatoshi Tohata, Katsutoshi Ara, Katsuya Ozaki, Kouji Nakamura

BIOTECHNOLOGY LETTERS, 33(9) 1847-1852, Sep, 2011 Peer-reviewed
Salt tolerance of a Sinorhizobium meliloti strain isolated from dry lands: growth capacity and protein profile changes

Moncef Mrabet, Kais Zribi, Haythem Mhadhbi, Naceur Djebali, Ridha Mhamdi, Mohamed Elarbi Aouani, Kouji Nakamura

ANNALS OF MICROBIOLOGY, 61(2) 361-369, Jun, 2011 Peer-reviewed
Propeptide of Bacillus subtilis amylase enhances extracellular production of human interferon-alpha in Bacillus subtilis

Hiroshi Kakeshita, Yasushi Kageyama, Katsutoshi Ara, Katsuya Ozaki, Kouji Nakamura

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 89(5) 1509-1517, Mar, 2011 Peer-reviewed
A novel toxin regulator, CPE1446-CPE1447 protein heteromeric complex controls toxin genes in Clostridium perfringens.

Obana, N, Nakamura Kouji

Journal of Bacteriology, 193(17) 4417-4424, Jan, 2011 Peer-reviewed
Enhanced Extracellular Production of Heterologous Proteins in Bacillus subtilis by Deleting the C-terminal Region of the SecA Secretory Machinery

Hiroshi Kakeshtia, Yasushi Kageyama, Katsutoshi Ara, Katsuya Ozaki, Kouji Nakamura

MOLECULAR BIOTECHNOLOGY, 46(3) 250-257, Nov, 2010 Peer-reviewed
Enhanced Extracellular Production of Heterologous Proteins in Bacillus subtilis by Deleting the C-terminal Region of the SecA Secretory Machinery (vol 46, pg 250, 2010)

Hiroshi Kakeshita, Yasushi Kageyama, Katsutoshi Ara, Katsuya Ozaki, Kouji Nakamura

MOLECULAR BIOTECHNOLOGY, 46(3) 320-320, Nov, 2010
Stabilization of Clostridium perfringens collagenase mRNA by VR-RNA-dependent cleavage in 5' leader sequence

Nozomu Obana, Yu Shirahama, Kimihiro Abe, Kouji Nakamura

MOLECULAR MICROBIOLOGY, 77(6) 1416-1428, Sep, 2010 Peer-reviewed
Effects of Depletion of RNA-Binding Protein Tex on the Expression of Toxin Genes in Clostridium perfringens

Kimihiro Abe, Nozomu Obana, Kouji Nakamura

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 74(8) 1564-1571, Aug, 2010 Peer-reviewed
Stabilization of Clostridium perfringens collagenase mRMA by VR-RNA-dependent cleavage in 5' leader sequence.

Nakamura,Kouji

Molecular Microbiology, 77(6) 1416-1428, Jul, 2010 Peer-reviewed
Expression, crystallization and preliminary crystallographic analysis of RNA-binding protein Hfq (YmaH) from Bacillus subtilis in complex with an RNA aptamer

Seiki Baba, Tatsuhiko Someya, Gota Kawai, Kouji Nakamura, Takashi Kumasaka

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 66(Part 5) 563-566, May, 2010 Peer-reviewed
Effects of deletion of an RNA-binding protein Tex on expression of toxin genes in Clostridium perfringens.

Abe, K, Obana, N, Nakamura, K

Bioscience, Biotechnology and Biochemistry, 74(8) 1564-1571, Jan, 2010 Peer-reviewed

Books and Other Publications

枯草菌のミニマムゲノムファクトリー-枯草菌MGFを用いた異種タンパク質生産技術、微生物機能を活用した革新的生産技術の最前線-ミニマムゲノムファクトリーとシステムバイオロジー

荒勝俊, 尾崎克也, 掛下大視, 中村幸治, 山根國男, 児玉武子, 関口順一, 門屋りょう介, 森本拓也, 小笠原直毅

シーエムシー, Dec, 2007
非翻訳型RNA(ncRNA）

中村, 幸治

RNA工学の最前線（シーエムシー）, Jan, 2006
真正細菌におけるnon-coding RNAによる遺伝子発現制御機構．

中村幸治, 安藤吉成

機能性RNA（クバプロ）, Jan, 2006
２１世紀型バイオプロスペクティング

宮崎均, 礒田博子, 安部征雄, 中村幸治 (Role: Supervisor (editorial))

化学と生物（日本農芸化学会）, Jan, 2005
小さなRNAの大きな仕事

中村幸治 (Role: Supervisor (editorial))

化学, Jan, 2003
試料の発現・調製法～微生物での発現～

中村, 幸治

ポストシークエンスタンパク質実験法２（東京化学同人）, Jan, 2002
真正細菌SRP RNAのタンパク質分泌・翻訳過程における多機能性についての研究

中村幸治 (Role: Supervisor (editorial))

日本農芸化学会誌, Jan, 2002
枯草菌におけるシグナル伝達、形質転換、蛋白質分泌

小倉光雄, 田中暉夫, 山根國男, 熊野みゆき, 佐野和良, 広瀬績, 中村幸治 (Role: Supervisor (editorial), Signal transduction, competence development and protein secretion in Bacillus subtilis)

蛋白質・核酸・酵素, Jan, 1999
食品安全性辞典

中村, 幸治

共立出版, Jan, 1998
真正細菌SRP RNAの多機能性

中村幸治, 山根國男 (Role: Supervisor (editorial))

日本生化学会誌, Jan, 1997
細菌性シグナル認識粒子SRP-構造・機能の哺乳類SRPとの比較

中村幸治, 高松宏治, 山根國男 (Role: Supervisor (editorial))

蛋白質・核酸・酵素, Jan, 1996
枯草菌における蛋白質分泌系と分泌装置

山根國男, 中村幸治 (Role: Supervisor (editorial))

日本農芸化学会誌, Jan, 1995
タンパク質分泌系におけるシグナルペプチドの機能

中村, 幸治

微生物の機能開発-バイオ研究の最前線（東大出版会）, Jan, 1992
Signal peptide cleavage and processing of artificial extracellular hybrid alpha-amylase in Bacillus subtilis

Yamane;K, Nakamura;K, Takamatsu, H, 中村, 幸治

In Bacillus subtilis Molecular genetics and biotechnology application (Academic Press), Jan, 1990
Secretion activity of the Bacillus subtilis alpha-amylase signal peptides with different lengths in Bacillus subtilis and Escherichia coli

Ohmura;K, Shiroza;T, Nakamura;K, Yamane;K, 中村, 幸治

In Bacillus subtilis Molecular genetics and biotechnology, Jan, 1986
Bacillus subtilis secretion vectors for proteins and oligopeptides constructed from Bacillus subtilis alpha amylase genes.

中村幸治

Molecular Biology of Microbial Differentiation (American Society for Microbiology), Jan, 1985

Professional Memberships

- Present

THE RNA SOCIETY OF JAPAN
- Present

RNA Society
- Present

The Molecular Biology Society of Japan
- Present

JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY

Research Projects

「微生物機能を活用した高度製造基盤技術開発」の関する研究（高性能宿主細胞創製技術の開発）

国内共同研究, Apr, 2009 - Mar, 2011
枯草菌による物質生産系に関する基盤技術に関する研究

学内共同研究, Aug, 2008 - Aug, 2009
生物機能を活用した生産プロセスの基盤技術開発

国内共同研究, Apr, 2005 - Mar, 2009
歯周病菌が生産するプロテアーゼの生産システムの開発とこれを利用した診断薬の開発

国内共同研究, ライオン（株）, Apr, 2005 - Mar, 2007
生物機能を活用した生産プロセスの基盤技術の開発

国内共同研究, 財団法人バイオインダストリー協会, Apr, 2004 - Mar, 2005
納豆製造過程における精製タンパク質のプロテオーム解析

国内共同研究, タカノフーズ, Apr, 2004 - Mar, 2005
Analyis of network on transcription and translation directed by small RNAs.
Structural analysis of secretory machinary, signal recognition particle(SRP)
環境汚染物質応答性遺伝子の解析