2000年5月
Cell-dependent requirement of human immunodeficiency virus type 1 gp41 cytoplasmic tail for Env incorporation into virions
JOURNAL OF VIROLOGY
- ,
- ,
- 巻
- 74
- 号
- 10
- 開始ページ
- 4891
- 終了ページ
- 4893
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1128/JVI.74.10.4891-4893.2000
- 出版者・発行元
- AMER SOC MICROBIOLOGY
Growth kinetics in lymphocytic H9 and M8166 cells of two mutants of human immunodeficiency virus type 1 (HIV-1) with deleted gp41 cytoplasmic tails were examined. While the mutant viruses designated CTdel-44 and CTdel-144 were able to grow in M8166 cells, they were unable to grow in H9 cells. Transfection and single-round infectivity assays demonstrated that they are defective in the early phase of viral replication in H9 cells. Analysis of the mutant virions revealed drastically reduced incorporation of Env gp120 (compared with the incorporation of wild-type virions) in H9 cells but normal incorporation in M8166 cells. These results indicate that the HIV-1 cytoplasmic tail of gp41 determines virus infectivity in a cell-dependent manner by affecting incorporation of Env into virions and suggest the involvement of a host cell factor(s) in the Env incorporation.
- リンク情報
- ID情報
-
- DOI : 10.1128/JVI.74.10.4891-4893.2000
- ISSN : 0022-538X
- eISSN : 1098-5514
- Web of Science ID : WOS:000086629400052