False smut is a serious disease affecting rice production worldwide. Initial infection of rice seedlings by Villosiclava virens was clarified using axenically cultured chlamydospores to inoculate rice roots. Chlamydospores were found on rice roots at 1 day post inoculation (dpi), and were germinating at 1-4 dpi. At 4 dpi, the infection germ tube had invaded the intercellular space between epidermal rice root cells. Between 5 and 11 dpi, branching and fusion-like structures were observed that may contribute to the establishment of the hyphal network on the root surface.