2016年9月1日
A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite
Biochemistry and Biophysics Reports
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- 巻
- 7
- 号
- 開始ページ
- 240
- 終了ページ
- 245
- 記述言語
- 英語
- 掲載種別
- 研究論文（学術雑誌）
- DOI
- 10.1016/j.bbrep.2016.06.020
- 出版者・発行元
- Elsevier B.V.
3,4-Dihydroxyphenylacetic acid (DOPAC) is one of the colonic microflora-produced catabolites of quercetin 4'-glucoside (Q4'G). Although the interaction of DOPAC with cellular proteins might be involved in its biological activity, the actual proteins have not yet been identified. In this study, we developed a novel tag-free DOPAC probe to label the targeted proteins by the copper(I)-catalyzed azide alkyne cycloaddition (CuAAC) and verified its efficacy. Various labeled proteins were detected by the DOPAC probe with the azide labeled biotin and a horseradish peroxidase (HRP)-streptavidin complex. Furthermore, a pulldown assay identified Keap1 and aryl hydrocarbon receptor (AhR) as the target proteins for the phase 2 enzyme up-regulation.
- リンク情報
- ID情報
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- DOI : 10.1016/j.bbrep.2016.06.020
- ISSN : 2405-5808
- SCOPUS ID : 84978982747